ArticleNucleic acids research2025
Bacterial RecD2 is a processive single-stranded DNA translocase with strand-switching capacity at DNA forks.
Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed.
- A superfamily 1 helicase translocates on double-stranded DNA.bioRxiv : the preprint server for biology · 2026Article
- Genetic dissection of DNA damage tolerance in Bacillus subtilis: RecA and recombination functions regulate translesion synthesis.Nucleic acids research · 2026Article
- RPA directly stimulates Mer3 helicase processivity to ensure normal crossover formation in meiosis.Nature communications · 2026Article
- Structural basis for fork reversal and RAD51 regulation by the SCF ubiquitin ligase complex of F-box helicase 1.Nature communications · 2026Article
- Mechanisms of chromosomal DNA replication in Escherichia coli and Bacillus subtilis.FEMS microbiology reviews · 2026Review
- Hallmarks of DNA replication stress responses in Escherichia coli and Bacillus subtilis.FEMS microbiology reviews · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
RecD2 is a superfamily 1B helicase involved in DNA replication and repair, modulating replication restart, fork progression, and RecA recombinase activity. In this work, we have characterized the functions of Bacillus subtilis RecD2 using biochemical and single-molecule approaches. ATPγS binding and low MgCl2 concentrations enhance DNA association, with a preference for forked structures and unstructured DNA longer than 30 nucleotides. RecD2 binds to end-less single-stranded DNA stretched at 8-20 pN and translocates through ATP hydrolysis over long distances (>20 kb) with 5'-3' polarity at high rates. RecD2 shows limited unwinding activity on fork structures, strongly dependent on protein concentration and duplex length, reflecting low processivity. However, processivity improves significantly when force is applied to the translocating strand or unwound DNA ends, enabling the unwinding of thousands of base pairs at rates up to 160 bp/s. Single-molecule assays reveal frequent strand switching on fork substrates, resulting in a non-productive cycle of unwinding and rewinding, likely mediated by the N-terminal domain. This behavior explains the low helicase activity observed in bulk assays. We propose that regulation of strand-switching activity may be relevant for RecD2's in vivo function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.