ArticleFood science & nutrition2025
Development of a New Purity Certified Reference Material of Gamma Linolenic Acid Methyl Ester.
Article in Food science & nutrition, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
In the study, a new purity certified reference material (CRM) of gamma-linolenic acid methyl ester (GLA-ME), designated as (GBW (E) 091214), was first developed in accordance with ISO Guides 17034 and 35. The research involved comprehensive investigations into the preparation, structure determination, characterization, homogeneity testing, stability testing, and uncertainty evaluation of the GLA-ME purity CRM. It was prepared by the preparative high-performance liquid chromatography (Pre HPLC) and molecular distillation (MD) method using evening primrose oil (EPO) as a raw material. The identity of GLA-ME was confirmed using high-resolution mass spectrometry (HRMS), nuclear magnetic resonance (NMR), and infrared spectrophotometry (IR). The purity of GLA-ME was accurately determined by the mass balance (MB) and further validated via quantitative nuclear magnetic resonance (
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