Chromosome-scale genome assembly of B10.RIII, an autoimmune susceptible mouse strain.

Vijayaraj Nagarajan, Yingyos Jittayasothorn, Guangpu Shi, Reiko Horai, Rachel R Caspi

Abstract readPreprint

In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

5 authors.

Vijayaraj NagarajanLaboratory of Immunology, National Eye Institute, National Institutes of Health, 10 Center Drive, 10/10N248, Bethesda, MD 20892-1857, USA.ORCID 0000-0003-2455-7172

Yingyos JittayasothornLaboratory of Immunology, National Eye Institute, National Institutes of Health, 10 Center Drive, 10/10N248, Bethesda, MD 20892-1857, USA.

Guangpu ShiLaboratory of Immunology, National Eye Institute, National Institutes of Health, 10 Center Drive, 10/10N248, Bethesda, MD 20892-1857, USA.

Reiko HoraiLaboratory of Immunology, National Eye Institute, National Institutes of Health, 10 Center Drive, 10/10N248, Bethesda, MD 20892-1857, USA.

Rachel R CaspiLaboratory of Immunology, National Eye Institute, National Institutes of Health, 10 Center Drive, 10/10N248, Bethesda, MD 20892-1857, USA.

Funding

Understanding the microbial requirements for colonization and immunogenicity of commensal bacteria at the ocular surfaceR01EY032482 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI ST LEGER, ANTHONY J · 2021 to 2025

$2.0M

NEI NIH HHS R01 EY032482

6 · The paper itself

Abstract

Background: A vast majority of potential drug candidates are initially tested in mice before human trials, resulting in life-saving treatments and preventive measures. Hundreds of autoimmune disorders, including arthritis, lupus etc., affect millions of people all over the world. Studying those disorders using mouse models is crucial for understanding the disease mechanisms. Knowing the genome sequence of these mouse models would help with those vital studies. B10.RIII is one of the most susceptible mouse strains used as a model to understand autoimmune diseases but did not have its genome sequenced until now. Findings: In this study, we generated the first, high-quality chromosome level genome sequence of B10.RIII, using a combination of long read, short read and optical mapping techniques. The B10.RIII genome sequence scored higher than the reference mouse genome both in assembly completeness and assembly quality. The B10.RIII genome annotation based on the reference genome identified about 98% of the known reference genes. Conclusions: We believe the availability of B10.RIII genome sequence will help advance the understanding of autoimmune diseases, giving the researchers a better idea on its unique genetic makeup. Comparative studies of B10.RIII with other mouse models could also significantly enhance the power of these models towards their clinical applications.

Indexed as

assemblyautoimmuneB10.RIIIgenome sequencemouse model

Identifiers

PMID40475550

DOI10.1101/2025.05.16.654505

PMCPMC12139855

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.