Evidence map›Paper›PMID 40475461›Full record

ArticlebioRxiv : the preprint server for biology2025

Engineering precision zebrafish alleles of human disease.

Holly R Thomas, Bradley K Yoder, Matthew S Alexander, John M Parant

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Holly R ThomasDepartment of Cell, Developmental and Integrative Biology, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA.
Bradley K YoderDepartment of Cell, Developmental and Integrative Biology, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA.
Matthew S AlexanderDepartment of Pediatrics, Division of Neurology at University of Alabama at Birmingham Heersink School of Medicine and Children's of Alabama, AL, USA.ORCID 0000-0001-7406-5171
John M ParantDepartment of Cell, Developmental and Integrative Biology, Heersink School of Medicine, University of Alabama at Birmingham, AL, USA.ORCID 0000-0001-9084-5098

Funding

UAB Pilot Center for Precision Animal Modeling (C-PAM) - Resource and Service SectionU54OD030167 · OD · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Bradley K. Yoder · 2020 to 2026
$15.3M
UAB Childhood Cystic Kidney Disease Core Center (UAB-CCKDCC) - Therapeutic Development and Screening ResourceU54DK126087 · NIDDK · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Brittany Nicole Lasseigne · 2020 to 2026
$6.7M
Deciphering How Esco2 Loss Acts as a Penetrance ModifierR01CA216108 · NCI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI PARANT, JOHN M · 2017 to 2021
$1.9M
NCI NIH HHS R01 CA216108NIDDK NIH HHS U54 DK126087NIH HHS U54 OD030167
6 · The paper itself

Abstract

Animal models of human diseases are an essential component of understanding disease pathogenesis and serve as preclinical models for therapeutic evaluation. Recently human patient genome sequencing has defined unique patient variants that result in disease states with different phenotypes than those observed with null alleles. The UAB Center for Precision Animal Modeling (CPAM) serves to analyze patient variant pathogenicity and disease mechanisms through the generation of animal models. We have optimized a zebrafish gene editing platform to successfully generate 11 patient variants (first round: NF1 R1276Q, NF1 G484R, VMA21 G55V, SPOP D144N, SGO1 K23E, Pex10 H310D, and FKRP C318Y; second round: NF1 R681*, NF1 M992del, P53 R175H, and PKD2 L656W) and 1 research allele (

Indexed as

CRIPSR/Cas9Genome Editinghigh-resolution melting curve analysis (HRMA)Inference of CRISPR Edits (ICE)Oligo directed homology directed repair (HDR)Precision animal modelsZebrafish

Identifiers

PMID40475461
PMCPMC12139734

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.