ArticleThe New phytologist2025
From glomalin to glomalose: unraveling the molecular identity of the MAb32B11 antigen.
Article in The New phytologist, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The trial behind it
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Who cites it
4 citing papers in PubMed.
- How can long-term cold storage affect the content of glomalin-related soil proteins from an arbuscular mycorrhizal inoculum?Antonie van Leeuwenhoek · 2026Article
- Arbuscular mycorrhizal fungi mediated biotic carbon pump in alpine meadows of the Qinghai Tibetan Plateau under a warming and wetting climate: patterns and mechanism.FEMS microbiology ecology · 2026Review
- Fungal highways to water: Mechanisms of drought tolerance in arbuscular and ectomycorrhizal symbioses.Mycorrhiza · 2026Review
- Impact of forest degradation on soil properties in the Peruvian Amazon.Carbon balance and management · 2026Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Glomalin, a substance produced by arbuscular mycorrhizal (AM) fungi, has well-documented benefits for plant and soil health, including water retention and soil aggregation. Glomalin quantification has been performed by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody, MAb32B11, that has been described as targeting a heat shock protein 60 (RiHSP60). In this study, we re-examined the molecular nature of the antigen recognized by MAb32B11. MAb32B11 did not cross-react with the RiHSP60 polypeptide. Glomalin extracts of Rhizophagus irregularis showed strong and dose-dependent cross-reactivity with MAb32B11 even when protein levels were undetectable, raising doubts about the proteinaceous nature of the antigen. Protease treatments of glomalin extracts did not affect the ELISA signal. However, treatment with periodate, which degrades polysaccharides, significantly reduced the signal. A strong correlation between carbohydrate content and the ELISA signal was observed in glomalin extracts. These findings indicate that MAb32B11 recognizes a carbohydrate, likely originating from cell walls of AM fungi. Further analysis of glomalin extracts using size exclusion chromatography suggests that the epitope of MAb32B11 is a complex carbohydrate in the size range of 511-600 kDa. Understanding the true nature of glomalin will enhance our ability to quantify it accurately and leverage its agricultural benefits.
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