Evidence map›Paper›PMID 40474232›Full record

ArticleBMC medical genomics2025

Chromosomal instability by low-coverage whole-genome sequencing assay predicts prognosis in bladder cancer patients underwent radical cystectomy.

Qi Ding, Hailiang Zhu, Bo Fan, Lisheng Wang, Xiaohua Jin, Cheng Cao, Ying Shi, Zhijiang Fan, Wenjian Tu, Feng Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC medical genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Qi Ding *Department of Urology, The First People's Hospital of Changshu, The Changshu Hospital Affiliated to Soochow University, 1 Shuyuan Street, Changshu, 215500, China.
Hailiang Zhu *Department of Urology, The First People's Hospital of Changshu, The Changshu Hospital Affiliated to Soochow University, 1 Shuyuan Street, Changshu, 215500, China.
Bo FanDepartment of Urology, The First People's Hospital of Changshu, The Changshu Hospital Affiliated to Soochow University, 1 Shuyuan Street, Changshu, 215500, China. fanbo_cs@sina.com.
Lisheng WangDepartment of Urology, The First People's Hospital of Changshu, The Changshu Hospital Affiliated to Soochow University, 1 Shuyuan Street, Changshu, 215500, China.
Xiaohua JinDepartment of Urology, The First People's Hospital of Changshu, The Changshu Hospital Affiliated to Soochow University, 1 Shuyuan Street, Changshu, 215500, China.
Cheng CaoDepartment of Urology, The First People's Hospital of Changshu, The Changshu Hospital Affiliated to Soochow University, 1 Shuyuan Street, Changshu, 215500, China.
Ying ShiDepartment of Gastroenterology, The First People's Hospital of Changshu, The Changshu Hospital Affiliated to Soochow University, 1 Shuyuan Street, 215500, Changshu, China.
Zhijiang FanDepartment of Urology, The First People's Hospital of Changshu, The Changshu Hospital Affiliated to Soochow University, 1 Shuyuan Street, Changshu, 215500, China.
Wenjian TuDepartment of Urology, The First People's Hospital of Changshu, The Changshu Hospital Affiliated to Soochow University, 1 Shuyuan Street, Changshu, 215500, China.
Feng LiDepartment of Urology, The First People's Hospital of Changshu, The Changshu Hospital Affiliated to Soochow University, 1 Shuyuan Street, Changshu, 215500, China.

Funding

Clinical Key Subject Construction Project Foundation of Changshu CSZDXK202001Introduction Project of Clinical Medicine Expert Team for Changshu CSYJTD202001the Changshu Medical Science and Technology Youth Program cswsq202102the Youth Science Funding Project of Suzhou KJXW2020063
6 · The paper itself

Abstract

purposeTo investigate chromosomal instability (CIN) in tumor tissue from radical bladder resection and to evaluate whether it can be used as a biomarker for the molecular typing of (BC).

methodsDNA was extracted from formalin-fixed paraffin-embedded samples of 50 BC patients who were followed up to March 23 2023 using the Qiagen nucleic acid kits. We analyzed CIN in tumor of bladder by low-coverage whole genome sequencing (LC-WGS). Kaplan-Meier log-rank test was used to perform survival analysis. The association between variables and overall and progression-free survival was analyzed using the Cox proportional hazards model.

resultsThere were 44 genome segments with statistically significant changes in copy number. CIN was significantly correlated with tumor stage, lymph node metastasis, relapse and survival status. Patients with high CIN were found to have a worse survival, with a median overall survival (OS) of 15 months. In addition, patients with high CIN were more likely to relapse, with a median progression-free survival (PFS) of 7 months. Patients with low CIN showed better OS and PFS. However, there was no significant difference in OS and PFS between T2 and T3-T4 patients. Multivariate cox regression analysis showed that high CIN was an independent predictor of OS, and high CIN and muscle invasion were independent predictors of PFS. Furthermore, patients with abnormal copy number of a single chromosome also had a poor prognosis, with a median survival of 14-30 months for OS and 5-10 months for PFS, while negative patients had a better prognosis.

conclusionCIN was significantly correlated with tumor stage, lymph node metastasis, relapse and survival status of BC. Patients with high CIN or abnormal copy numbers of a single chromosome have a poor prognosis. CIN might be better than T stage in predicting the prognosis of patients with BC. Molecular typing of CIN can be used as an independent prognostic factor for BC.

Indexed as

Chromosomal InstabilityCystectomyUrinary Bladder NeoplasmsWhole Genome SequencingAdultAgedFemaleHumansMaleMiddle AgedPrognosisBiomarkerBladder cancerChromosomal instabilityLow-coverage whole genome sequencingPrognosis

Identifiers

PMID40474232
PMCPMC12142956

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.