ArticleCommunications chemistry2025
Molecular dynamics guided identification of a brighter variant of superfolder Green Fluorescent Protein with increased photobleaching resistance.
Article in Communications chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The trial behind it
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Who cites it
5 citing papers in PubMed.
- Barrel Shape and Chromophore Rigidity Predict Fluorescent-Protein Photophysics.Journal of chemical information and modeling · 2026Article
- Metal-Enhanced Fluorescence of Nanocomplexes.Materials (Basel, Switzerland) · 2026Article
- Physical Mechanisms of an Unconventional Green Fluorescent Protein Indicator for Chloride.The journal of physical chemistry. B · 2026Article
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Structure, Function and Dynamics of mCoral, a pH-Responsive Engineered Variant of the mCherry Fluorescent Protein with Improved Hydrogen Peroxide Tolerance.International journal of molecular sciences · 2025Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Fluorescent proteins (FPs) are a crucial tool for cell imaging, but with developments in fluorescence microscopy and researcher requirements there is still a need to develop brighter versions that remain fluorescent for longer. Using short time-scale molecular dynamics-based modelling to predict changes in local chromophore interaction networks and solvation, we constructed an Aequorea victoria GFP (avGFP) variant called YuzuFP that is 1.5 times brighter than the starting superfolding variant (sfGFP) with a near 3-fold increased resistance to photobleaching in situ. YuzuFP contained a single mutation that replaces the chromophore interacting residue H148 with a serine. Longer time scale molecular dynamics revealed the likely mechanism of action: S148 forms more persistent H-bond with the chromophore phenolate group and increases the residency time of an important water molecule. As demonstrated by live cell imaging, YuzuFP not only offers a timely upgrade as a useful green-yellow avGFP for cell imaging applications over longer time scales, but it also provides a basic scaffold for future avGFP engineering efforts.
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Registered trials
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