Automated tissue dissociation for the establishment of human intestinal organoids.

Woo Jin Yang, Eva Blahusova, Komal Nayak, Róisín M Owens, Matthias Zilbauer

Abstract read

In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Woo Jin YangCambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.

Eva BlahusovaCambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.

Komal NayakCambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.

Róisín M OwensDepartment of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK. rmo37@cam.ac.uk.

Matthias ZilbauerCambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. mz304@cam.ac.uk.

Funding

Cytiva Studentship refG117597Helmsley Charitable Trust, funded by The Leona M and Harry B Helmsley Charitable Trust G118500

6 · The paper itself

Abstract

Human intestinal Organoids (HIOs) may be set up from primary human tissue following dissociation, release, and culture of intestinal LGR5 + stem cells. Here, we characterise the use of a semi-automated mechanical tissue dissociation technique, which demonstrates an expedited workflow and streamlined protocol, standardising key aspects of the process while consistently maintaining a high level of cultured organoid viability.

Indexed as

IntestinesOrganoidsAutomationCell Culture TechniquesHumansIntestinal MucosaReceptors, G-Protein-CoupledStem CellsLGR5 protein, humanReceptors, G-Protein-CoupledAutomationHuman intestinal organoidsTissue dissociation

Identifiers

PMID40473694

DOI10.1038/s41598-025-03905-9

PMCPMC12141665

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.