Evidence map›Paper›PMID 40472572›Full record

ArticleDiagnostic microbiology and infectious disease2025

A pooled testing strategy for enterovirus D68 to facilitate local, resource-conserving surveillance.

Madeline E Spradley, Timothy Williams, Rendie McHenry, Laura L Short, Dalton J Nelson, James D Chappell, Natasha Halasa, Frederick R Haselton, Jonathan E Schmitz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostic microbiology and infectious disease, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Madeline E SpradleyDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
Timothy WilliamsDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA; Current institution: Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Rendie McHenryDepartment of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA.
Laura L ShortDepartment of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA.
Dalton J NelsonDepartment of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.
James D ChappellDepartment of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA.
Natasha HalasaDepartment of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA; Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center, Nashville, TN, USA.
Frederick R HaseltonDepartment of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.
Jonathan E SchmitzDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA; Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center, Nashville, TN, USA; Department of Urology, Vanderbilt University Medical Center, Nashville, TN, USA. Electronic address: jonathan.e.schmitz@vumc.org.

Funding

Point-of-Care RT-PCR System to Inform COVID-19 and Respiratory Illness DecisionsR01AI157827 · NIAID · VANDERBILT UNIVERSITY · PI Frederick R Haselton, Jonathan E Schmitz · 2020 to 2026
$4.7M
NIAID NIH HHS R01 AI157827
6 · The paper itself

Abstract

Pooled testing represents a resource-conserving diagnostic strategy, and we propose enterovirus-D68 (EV-D68) as an attractive target for pooling (especially locally). To validate an RT-qPCR-based approach, retrospective pools were contrived from EV-D68-positive nasopharyngeal specimens and non-EV-D68-enterovirus-positive specimens. Five/nine-fold pools demonstrated 90.9 %/88.6 % sensitivity versus-unpooled testing (no false-positives), with commensurate results observed prospectively.

Indexed as

Enterovirus D, HumanEnterovirus InfectionsMolecular Diagnostic TechniquesSpecimen HandlingChild, PreschoolEpidemiological MonitoringHumansNasopharynxPooled TestingReal-Time Polymerase Chain ReactionRetrospective StudiesSensitivity and SpecificityEnterovirus D68Outbreak surveillancePooled testingRespiratory virusRT-qPCR

Identifiers

PMID40472572
PMCPMC13521326

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.