Evidence map›Paper›PMID 40471565›Full record

ArticleChemistry (Weinheim an der Bergstrasse, Germany)2025

Convergent Approach Toward ADP-Ribosylated Peptides via a Chemoselective Phosphate Condensation.

Sven Wijngaarden, Femke L A M van der Heijden, Cindy J Bogaart, Maria Siskou, Ioanna Tsoumani, Daisy J Robinson, Gijsbert A van der Marel, Jeroen D C Codée, Herman S Overkleeft, Dmitri V Filippov

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Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

10 authors.

Sven WijngaardenLeiden Institute of Chemistry, Leiden University, Einsteinweg 55, Leiden, 2333 CC, The Netherlands.
Femke L A M van der HeijdenLeiden Institute of Chemistry, Leiden University, Einsteinweg 55, Leiden, 2333 CC, The Netherlands.
Cindy J BogaartLeiden Institute of Chemistry, Leiden University, Einsteinweg 55, Leiden, 2333 CC, The Netherlands.
Maria SiskouLeiden Institute of Chemistry, Leiden University, Einsteinweg 55, Leiden, 2333 CC, The Netherlands.
Ioanna TsoumaniLeiden Institute of Chemistry, Leiden University, Einsteinweg 55, Leiden, 2333 CC, The Netherlands.
Daisy J RobinsonLeiden Institute of Chemistry, Leiden University, Einsteinweg 55, Leiden, 2333 CC, The Netherlands.
Gijsbert A van der MarelLeiden Institute of Chemistry, Leiden University, Einsteinweg 55, Leiden, 2333 CC, The Netherlands.
Jeroen D C CodéeLeiden Institute of Chemistry, Leiden University, Einsteinweg 55, Leiden, 2333 CC, The Netherlands.
Herman S OverkleeftLeiden Institute of Chemistry, Leiden University, Einsteinweg 55, Leiden, 2333 CC, The Netherlands.
Dmitri V FilippovLeiden Institute of Chemistry, Leiden University, Einsteinweg 55, Leiden, 2333 CC, The Netherlands.ORCID https://orcid.org/0000-0002-6978-7425

Funding

Dutch Research Council OCENW.KLEIN.497Nederlandse Organisatie voor Wetenschappelijk Onderzoek OCENW.KLEIN.497
6 · The paper itself

Abstract

The preparation of well-defined ADP-ribosylated peptides is essential for studying the functional implications of this post-translational modification. While methodologies exist for the chemical synthesis of short oligo-ADPr fragments and mono-ADP-ribosylated peptides separately, combining the two distinct chemistries, required to assemble them, has remained challenging. In this research, we employ a methodology for the regio- and chemoselective condensation of two phosphomonoesters to convergently install the pyrophosphate bond in ADP-ribosylated constructs. A diverse set of phosphoribosylated peptides, varying in the amino acid acceptor, was prepared and condensed with adenosine monophosphate to yield mono-ADP-ribosylated peptides. Furthermore, a solid-phase approach was developed to access a phosphoadenosyl-ADPr fragment, which was used to prepare a di-ADPr-peptide as the first example of a synthetic oligo-ADP-ribosylated peptide. Overall, the work presented here extends synthetic methodology for the preparation of well-defined ADP-ribosylated peptides.

Indexed as

Adenosine Diphosphate RibosePeptidesPhosphatesAdenosine MonophosphateADP-RibosylationProtein Processing, Post-TranslationalSolid-Phase Synthesis TechniquesAdenosine Diphosphate RiboseAdenosine MonophosphatePeptidesPhosphatesadenosine diphosphate ribosepeptidesphosphate condensationphosphorylationsolid‐phase synthesis

Identifiers

PMID40471565
PMCPMC12238907

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