Quantitative analysis of cancer cell morphology using digital holography technology under high temperature stimulation.

Zhuowen Chen, Xin Li, Penglong Li, Xiaojiang Wang, Lixia Zhen, Huanfei Wen, Zongmin Ma, Jun Tang, Jun Liu

Abstract read

In one paragraph

Article in Histochemistry and cell biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Zhuowen ChenSchool of Semiconductor and Physics, State Key Laboratory of Solid State Lighting, North University of China, Taiyuan, 030051, China.

Xin LiSchool of Instrument and Electronics, State Key Laboratory of Extreme Environment Optoelectronic Dynamic Testing Technology and Instrument, North University of China, Taiyuan, 030051, China.

Penglong LiSchool of Instrument and Electronics, State Key Laboratory of Extreme Environment Optoelectronic Dynamic Testing Technology and Instrument, North University of China, Taiyuan, 030051, China.

Xiaojiang WangSchool of Semiconductor and Physics, State Key Laboratory of Solid State Lighting, North University of China, Taiyuan, 030051, China.

Lixia ZhenSchool of Instrument and Electronics, State Key Laboratory of Extreme Environment Optoelectronic Dynamic Testing Technology and Instrument, North University of China, Taiyuan, 030051, China.

Huanfei WenSchool of Instrument and Electronics, State Key Laboratory of Extreme Environment Optoelectronic Dynamic Testing Technology and Instrument, North University of China, Taiyuan, 030051, China.

Zongmin MaSchool of Semiconductor and Physics, State Key Laboratory of Solid State Lighting, North University of China, Taiyuan, 030051, China.

Jun TangSchool of Semiconductor and Physics, State Key Laboratory of Solid State Lighting, North University of China, Taiyuan, 030051, China.

Jun LiuSchool of Instrument and Electronics, State Key Laboratory of Extreme Environment Optoelectronic Dynamic Testing Technology and Instrument, North University of China, Taiyuan, 030051, China.

Funding

The Basic Research Program of Shanxi Province 202203021222058The Dynamic Testing State Key Laboratory Fund 2410600045MZ

6 · The paper itself

Abstract

In cancer tissue diagnostic studies of clinical medicine, cell morphology is a key indicator for assessing the behavior of cellular physiological activities. Currently, the method of using thermotherapy as an adjunct to cancer treatment has gradually become a trend. Assessing the morphological characteristics of wound tissue cells during the hyperthermia process is particularly important for providing feedback on the therapeutic efficacy of hyperthermia-assisted treatment. Among the existing cell observation techniques, optical bright-field microscopy can only perform static observations of cells from a two-dimensional planar perspective. However, fluorescence microscopy suffers from issues such as phototoxicity and low temporal resolution. To address the aforementioned issues, this study introduces a quantitative analysis method based on digital holography to overcome these limitations. According to the mechanism of hyperthermia in cancer treatment, cancer cells exhibit morphological changes when exposed to elevated temperatures. Digital holography technology can effectively utilize light refractive indexes and phase differences to quantify the thickness and volume of cells. This study systematically evaluated the morphological changes in HeLa cells and human cervical epithelial cells (HCECs) under different temperature gradients (37-42 ℃ and 60 ℃). Continuous tracking of cell thickness and volume was achieved. The results revealed a unique morphological thermoresponsive process of the cells. The study determined the temperature threshold and exposure duration for high-temperature-induced effects in HeLa cells. The greater temperature sensitivity of HeLa cells compared with HCECs has been verified. This technology is expected to provide an effective means for evaluating morphological changes in cellular thermoresponses, offering novel insights for optimizing personalized cancer treatment regimens.

Indexed as

HolographyHot TemperatureNeoplasmsHeLa CellsHumansQuantitative Phase ImagingDigital holographyMorphological changeQuantitative analysisThermal response

Identifiers

PMID40471436

DOI10.1016/j.aquaculture.2021.737401

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.