Evidence map›Paper›PMID 40471428›Full record

ReviewOphthalmology and therapy2025

Lissamine Green in Ophthalmology: A Comprehensive Review of Diagnostic and Clinical Applications.

Stefano Barabino, Pasquale Aragona, Stefano Bonini, Emilia Cantera, Antonio Di Zazzo, Giuseppe Giannaccare, Andrea Leonardi, Giancarlo Montani, Alessandro Mularoni, Vincenzo Orfeo and 3 more

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Ophthalmology and therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Stefano BarabinoOcular Surface & Dry Eye Center, ASST Fatebenefratelli SACCO, Milan University, 20123, Milan, Italy. stebarabi@gmail.com.
Pasquale AragonaDepartment of Biomedical Sciences, Ophthalmology Clinic, University Hospital of Messina, Messina, Italy.
Stefano BoniniOphthalmology Campus Bio-Medico University, Rome, Italy.
Emilia CanteraIsraelitic Hospital, Rome, Italy.
Antonio Di ZazzoOphthalmology Campus Bio-Medico University, Rome, Italy.
Giuseppe GiannaccareDepartment of Surgical Sciences, Eye Clinic, University of Cagliari, Cagliari, Italy.
Andrea LeonardiDepartment of Neurosciences, Ophthalmology Unit, University of Padua, Padua, Italy.
Giancarlo MontaniDepartment of Mathematics and Physics, CeRca Lab, University of Salento, Lecce, Italy.
Alessandro MularoniOphthalmic Department, San Marino Public Hospital, Cailungo, Republic of San Marino.
Vincenzo OrfeoUnità Operativa di Oculistica Clinica Mediterranea, Naples, Italy.
Edoardo VillaniDepartment of Clinical Science and Community Health, University of Milan, Milan, Italy.
Fabrizio ZeriMaterials Science Department and COMiB Research Center, University of Milano Bicocca, Milan, Italy.
Maurizio RolandoEuropean Dry Eye Society, Genoa, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lissamine green (LG), a diagnostic dye that stains devitalized or damaged epithelial cells, is widely used to assess ocular surface integrity, enabling the detection of inflammation, epithelial defects, and conjunctival irregularities. To explore the diagnostic and clinical applications of LG in ophthalmology, focusing on its use for ocular surface diseases, a group of experts in ophthalmology and optometry participated in an advisory board to share their clinical practice experience with the use of LG. Building on the advisory board contents, this narrative review was based on a combination of expert opinions from the roundtable discussion and a comprehensive review of the current literature. This review highlights the clinical relevance of LG as a diagnostic tool in ocular surface disease and underscores the potential of newer formulations to enhance diagnostic accuracy. In particular, the review highlights the historical development of LG use in ophthalmology and its advantages over other dyes, especially in terms of patient comfort and safety, as well as specific clinical protocols for using LG in assessing dry eye disease severity and inflammatory responses. Additionally, the review examines recent advancements in LG formulations, which enhance their utility in clinical practice, and addresses safety considerations. Potential areas for future research are also discussed, particularly in developing standardized evaluation procedures using artificial intelligence.

Indexed as

Clinical protocolsContact lensesLissamine greenOcular surface stainingOphthalmologyTear film assessment

Identifiers

PMID40471428
PMCPMC12271041

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.