Evidence map›Paper›PMID 40471329›Full record

ArticleMolecular genetics and genomics : MGG2025

lncBNIP3 knockdown enhances bovine myoblast proliferation by modulating DNA replication and cell cycle pathways.

Meng Yang, Yishan Pang, Sayed Haidar Abbas Raza, Juntao Guo, Jianfang Wang, Gongwei Zhang, Sameerh Alsahafi, Majid Al-Zahrani, Fuyuan Zuo, Wenzhen Zhang

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In one paragraph

Article in Molecular genetics and genomics : MGG, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Meng Yang *College of Animal Science and Technology, Southwest University, No.160 College Road, Rongchang, 402460, P.R. China.
Yishan Pang *College of Animal Science and Technology, Southwest University, No.160 College Road, Rongchang, 402460, P.R. China.
Sayed Haidar Abbas RazaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, 510275, PR China.ORCID http://orcid.org/0000-0002-0961-1911
Juntao GuoCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China.
Jianfang WangCollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China.
Gongwei ZhangCollege of Animal Science and Technology, Southwest University, No.160 College Road, Rongchang, 402460, P.R. China.
Sameerh AlsahafiDepartment of Biology, Faculty of Science, Al-Baha University, P.O. Box 1988, Al-Baha, Al-Baha, 65527, Kingdom of Saudi Arabia.
Majid Al-ZahraniDepartment of Biological Sciences, College of Science and Art, King Abdulaziz University, Rabigh, Saudi Arabia.
Fuyuan ZuoCollege of Animal Science and Technology, Southwest University, No.160 College Road, Rongchang, 402460, P.R. China.
Wenzhen ZhangCollege of Animal Science and Technology, Southwest University, No.160 College Road, Rongchang, 402460, P.R. China. zwz0909@126.com.ORCID http://orcid.org/0000-0001-8315-1514

Funding

This work was supported by the Natural Science Foundation of Chongqing (CSTB2024NSCQ-MSX1261) and Fundamental Research Funds for the Central Universities (SWU-KQ24030). (CSTB2024NSCQ-MSX1261)
6 · The paper itself

Abstract

Myogenesis, a multistep process involving myoblast proliferation and differentiation, is critical for determining the economic value of beef cattle. While long noncoding RNAs (lncRNAs) are known to regulate myoblast proliferation, their specific mechanisms remain unclear. This study investigates the role of lncBNIP3 in bovine myoblast proliferation and examines the effects of its knockdown on cellular biological characteristics. Using quantitative real-time PCR (qRT-PCR), lncBNIP3 expression was observed to be higher in muscle tissues compared to other tissues in both 1-day-old and 24-month-old Qinchuan cattle. Knockdown of lncBNIP3 expression upregulated the mRNA levels of proliferation-related genes, as confirmed by qRT-PCR, and subsequently enhanced cellular proliferation, as demonstrated through EdU assays, flow cytometry, and CCK-8 analysis. Transcriptomic sequencing of myoblasts revealed that differentially expressed genes (DEGs) were significantly enriched in pathways associated with DNA replication and the cell cycle. Shared DEGs were primarily enriched in the minichromosome maintenance (MCM) gene family. Additionally, qRT-PCR and transcriptomic sequencing results revealed that the knockdown of lncBNIP3 expression significantly upregulated the mRNA levels of MCM family genes, including MCM2 and MCM3. Fluorescence activity assays further showed that lncBNIP3 knockdown significantly enhanced the promoter activities of MCM2 and MCM3. These findings suggest that interference with lncBNIP3 expression promotes the proliferation of bovine myoblasts, potentially through transcriptional regulation of the MCM gene family. This study provides novel insights into the regulatory functions of lncRNAs in muscle development.

Indexed as

Cell CycleDNA ReplicationMyoblastsRNA, Long NoncodingAnimalsCattleCell DifferentiationCell ProliferationGene Knockdown TechniquesMuscle DevelopmentRNA, Long NoncodingBovine myoblastsCell proliferationlncBNIP3MCM gene family

Identifiers

PMID40471329

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.