Evidence map›Paper›PMID 40470970›Full record

ArticleJournal of clinical microbiology2025

Inter-laboratory variability in cytomegalovirus DNA quantification: implications for standardization and clinical monitoring.

D Boutolleau, A-S L'Honneur, R Germi, B Chanzy, C Archimbaud-Jallat, C Rzadkowolski, J B Raimbourg, D Gauthier, V Thibault

Abstract read
In one paragraph

Article in Journal of clinical microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

D BoutolleauAP-HP. Pitié-Salpêtrière Hospital, Virology Department, National Reference Centre for Herpesviruses (Associated Laboratory), Sorbonne Université, Paris, France.
A-S L'HonneurDepartment of Virology, Cochin Hospital and University of Paris Cité, AP-HP, Paris, France.
R GermiLaboratoire de Virologie, CHU Grenoble, CNRS, CEA, IRIG IBS, University Grenoble Alpes, Saint-Martin-d'Hères, Auvergne-Rhône-Alpes, France.
B ChanzyCH Annecy Genevois, Épagny Metz-Tessy, Auvergne-Rhône-Alpes, France.
C Archimbaud-JallatService de Virologie CHU Clermont-Ferrand, Centre National de Reference des Enterovirus et Parechovirus, Clermont-Ferrand, France.
C RzadkowolskiBio-Rad Laboratories, Fort Worth, Texas, USA.
J B RaimbourgBio-Rad Laboratories, Marnes-La-Coquette, France.
D GauthierBio-Rad Laboratories, Irvine, California, USA.
V ThibaultCHU Rennes, Virology, Rennes, France.ORCID 0000-0001-6517-2901

Funding

Bio-Rad Laboratories
6 · The paper itself

Abstract

Cytomegalovirus (CMV) infection monitoring is a key element in the management of immunocompromised patients. CMV DNA quantification in plasma or whole blood is the best indicator for clinicians to adjust immunosuppressive or antiviral therapies. Despite the availability of internationally standardized material, the commutability of CMV quantification results across laboratories remains inadequate. To assess inter-laboratory variability in CMV DNA quantification, we conducted a blinded study in seven independent laboratories. Each participant received a panel of 92 specimens for CMV quantification using their routinely used standard platform. While quantifications were highly correlated and reproducible, large discrepancies were observed with differences up to 1.45 log

Indexed as

CytomegalovirusCytomegalovirus InfectionsDNA, ViralViral LoadHumansLaboratoriesReproducibility of ResultsDNA, Viralcalibrationextractionfragmentationimmunosuppressionviral load monitoring

Identifiers

PMID40470970
PMCPMC12239724

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.