Evidence map›Paper›PMID 40469104›Full record

ArticleiScience2025

Unveiling alternative splicing dynamics in activated T lymphocytes and their implications for immune checkpoint blockade efficacy.

Elad Zisman, Ori Stern, Reyut Lewis, Shay Tzaban, Thomas Eisenhaure, Sarah E Henrickson, Moshe Sade-Feldman, Shira Silberman-Klein, Galit Eisenberg, Nir Hacohen and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Dysregulation of U12-Type Splicing in Lupus Neutrophils.Arthritis & rheumatology (Hoboken, N.J.) · 2026
    Article
  2. Dysregulation of U12-Type Splicing in Lupus Neutrophils.bioRxiv : the preprint server for biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Elad ZismanThe Lautenberg Center for Immunology and Cancer Research, The Faculty of Medicine Hebrew University of Jerusalem, Jerusalem, Israel.
Ori SternThe Lautenberg Center for Immunology and Cancer Research, The Faculty of Medicine Hebrew University of Jerusalem, Jerusalem, Israel.
Reyut LewisThe Lautenberg Center for Immunology and Cancer Research, The Faculty of Medicine Hebrew University of Jerusalem, Jerusalem, Israel.
Shay TzabanThe Lautenberg Center for Immunology and Cancer Research, The Faculty of Medicine Hebrew University of Jerusalem, Jerusalem, Israel.
Thomas EisenhaureBroad Institute of MIT and Harvard, Division of Allergy Immunology, Boston, MA, USA.
Sarah E HenricksonDepartment of Pediatrics, the Children's Hospital of Philadelphia, Philadelphia, and Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Moshe Sade-FeldmanBroad Institute of MIT and Harvard, Division of Allergy Immunology, Boston, MA, USA.
Shira Silberman-KleinThe Lautenberg Center for Immunology and Cancer Research, The Faculty of Medicine Hebrew University of Jerusalem, Jerusalem, Israel.
Galit EisenbergThe Lautenberg Center for Immunology and Cancer Research, The Faculty of Medicine Hebrew University of Jerusalem, Jerusalem, Israel.
Nir HacohenBroad Institute of MIT and Harvard, Division of Allergy Immunology, Boston, MA, USA.
Keren YizhakTechnion Israel Institute of Technology, Haifa, Israel.
Michal LotemThe Lautenberg Center for Immunology and Cancer Research, The Faculty of Medicine Hebrew University of Jerusalem, Jerusalem, Israel.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alternative splicing (AS) generates diverse mRNA transcripts from a single gene, enhancing protein variety. This study examines AS during naive CD4 T cell activation, revealing that splicing events precede gene expression changes, evident as early as 6 h post-activation. Splicing alterations primarily impact pathways related to protein metabolism and mRNA processing, while gene expression dynamics affect immune regulatory genes. Notably, splicing isoforms favored soluble ectodomains of surface receptors, including CTLA4. CD8 T cells showed comparable patterns to CD4 cells, primarily differing in immune-related genes. Analysis of T cell-rich tumor samples from melanoma patients showed that AS events prevalent in the CD4 dataset correlated with positive responses to immune checkpoint inhibitors (ICIs). These findings suggest that activation-induced AS in T cells may play an immune regulatory role and be linked to ICI treatment outcomes.

Indexed as

Biological sciencesImmune responseImmunologyMolecular biology

Identifiers

PMID40469104
PMCPMC12135390

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.