ArticleIntegrative cancer therapies
In Vitro Anticancer Activity of Phytol on Human Non-Small Cell Lung Cancer A549 Cells.
Article in Integrative cancer therapies. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Phytol in Skin Care: From Multidimensional Pharmacological Mechanisms to Nanocarrier-Based Cosmetic Applications.International journal of molecular sciences · 2026Review
- Eucalyptus camaldulensis leaf extract enhances thalidomide efficacy in acute myeloid leukemia cells through modulation of mitochondrial energetics.BMC complementary medicine and therapies · 2026Article
- Vernodalin andBiomedicines · 2026Article
- From Gas Chromatography-Mass Spectrometry (GC-MS) to Network Pharmacology: System-Level Insights into the Multi-Target Biological Potential ofCurrent issues in molecular biology · 2026Article
- Tiny bryophytes: nature's hidden reservoirs of powerful anti-cancer compounds.The Plant cell · 2026Article
- Unveiling the Phytochemical Profile and Anti-Cancer Potential ofFood science & nutrition · 2025Article
- Targeting SPATS2 in lung adenocarcinoma: implications for prognosis and immune-based therapy.Discover oncology · 2025Article
- Antitumor Effects of Chemlali and Wild Olive Tree Extracts: Role in Cell Proliferation and Apoptosis in Prostate (PC3) and Breast (MDA-MB-231) Cancer Cell Lines.BioMed research international · 2025Article
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Authors and funding
4 authors.
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Abstract
backgroundNon-small cell lung cancer (NSCLC) is the most common type of lung cancer. Many researchers have previously reported that natural compounds from plants or Chinese Traditional Herbs have a potential to treat NSCLC. But it has not been reported that phytol can treat NSCLC. In this research, we first exposed this effect on A549 cells and researched the mechanism.
methodsIn order to evaluate whether phytol has a role in human NSCLC, a human non-tumoral bronchial epithelial cell line (NL20), adenocarcinomic human alveolar basal epithelial (A549) cell line, and NCI-H69 SCLC (H69) cell line were used for related experiments. After determining that phytol had no toxicity to NL20 cells, A549 cells, or H69 cells, the inhibitory effect of phytol on cancer cell related characteristics of cells were determined by luciferase assay, QRT-PCR, proliferation, invasion, and would healing cellular response experiments. Additionally, the quantification of apoptotic cells has been achieved through flow cytometry. Then, bioinformatics was used to establish a database to screen and speculate on phytol's corresponding targets in lung cancer. Finally, immunoblotting experiments were used to determine the specific pathways affected by phytol.
resultsTreatment with phytol at concentrations ranging from 0 to 80 µM for 24 hours was not cytotoxic to the A549 cells and H69 cells. Phytol inhibited AP-1-mediated and NF-κB-mediated luciferase activity in a dose-dependent manner in A549 cells, but not H69 cells. Additionally, phytol significantly inhibited the levels of MMP9, IL-6, VEGFA, IL-8, and NFKBIA in A549 cells, but had no significant effects on H69 cells. Phytol induced significant dose-dependent growth inhibitory effects on A549 cells. A significant decrease in colony formation and migration was observed. Bioinformatic and immunoblotting analysis indicated that phytol inhibited proliferation and migration of A549 cells through the PI3K-Akt signaling pathway.
conclusionsPhytol exhibits anticancer activity by inhibiting PI3K-Akt signaling pathway and may be applicable in the clinical prevention and treatment of lung cancer in the future.
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