Observational studyCritical care (London, England)2025
Performance of broad-spectrum targeted next-generation sequencing in lower respiratory tract infections in ICU patients: a prospective observational study.
Observational study in Critical care (London, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Quantitative interpretation models for targeted next-generation sequencing in lower respiratory tract infections: a multicenter prospective study.Respiratory research · 2026Article
- Broad-spectrum targeted next-generation sequencing: is it ready for routine deployment in intensive care units for severe pneumonia?Critical care (London, England) · 2026Article
- Application value of next generation sequencing technology for pathogen detection in patients with pulmonary infection and lung cancer.American journal of cancer research · 2026Article
- The clinical application of metagenomic next-generation sequencing for invasive pulmonary aspergillosis in neutropenic patients: a multicenter retrospective study in the ICU.Frontiers in cellular and infection microbiology · 2026Article
- Clinical evaluation of probe capture-based targeted next-generation sequencing in suspected infected pancreatic necrosis: a prospective pilot diagnostic study.BMC infectious diseases · 2025Article
- Associations of targeted next-generation sequencing with antimicrobial stewardship and clinical outcomes in pediatric lower respiratory tract infections.Respiratory research · 2025Article
- Ultra-broad hybrid capture-based targeted next-generation sequencing for sensitive plasma pathogen cfDNA detection in bloodstream infections.Journal of translational medicine · 2025Article
- Molecular diagnostic technology: beyond analytical accuracy.Critical care (London, England) · 2025Article
- Targeted next-generation sequencing using bronchoalveolar lavage fluid samples for diagnosing pulmonary infections: a single-center retrospective study.Frontiers in microbiology · 2025Article
- Precise pathogen detection and clinical characterization of bronchiectasis.Frontiers in cellular and infection microbiology · 2025Article
- Targeted next-generation sequencing for pediatric lower respiratory tract infections: a retrospective study.Frontiers in cellular and infection microbiology · 2025Article
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Authors and funding
18 authors.
Funding
Abstract
purposeTargeted next-generation sequencing (tNGS) has emerged as an advanced diagnostic technique. While tNGS is increasingly recognized as a valuable tool for detecting infections, its most relevant clinical indications remain underdefined. This study aimed to evaluate the clinical utility of tNGS for lower respiratory tract infections (LRTIs).
methodsWe conducted a prospective, observational study to evaluate the clinical diagnostic value of broad-spectrum targeted Next-Generation Sequencing (bstNGS) covering 1872 microorganisms in critically ill patients with LRTIs. We compared the microbial detection performance of bstNGS, mNGS, and traditional culture methods in bronchoalveolar lavage fluid (BALF). Additionally, we used the odds ratio (OR) from multiple logistic regression to assess the impact of relevant clinical variables on the detection of pathogens by bstNGS. We also examined the correlation between bstNGS pathogen detection results and clinical outcomes.
resultsBetween August 23, 2023, and April 24, 2024, samples from 150 patients were analyzed. bstNGS detected 96.33% and 91.15% of the microorganisms discovered by mNGS and culture respectively, and was capable of identifying microorganisms with even lower loads. According to the diagnostic criteria, bstNGS, mNGS, and culture methods detected pathogens in 87.33%, 82.00%, and 46.00% of the samples respectively. Moreover, the NGS methods demonstrated a stronger pathogen detection ability compared to culture (p < 0.05). Further comparing the diagnostic performance of the three methods, bstNGS exhibited higher diagnostic accuracy than both mNGS (90.67% vs 86.00%, p < 0.05) and culture (90.67% vs 49.33%, p < 0.0001). Multivariate analysis revealed that immunocompromise was associated with a lower efficiency of pathogen detection by bstNGS (p = 0.04), while other included clinical features had no significant correlation with bstNGS detection. Additionally, compared with patients in whom no pathogen was detected, patients in whom a pathogen was detected by bstNGS were associated with better outcomes of antibiotic treatment (89.68% vs. 62.50%; OR 7.53, 95% CI 1.41-45.30; p = 0.02).
conclusionThis study shows the effectiveness of bstNGS in detecting pathogens of LRTIs, as well as its value as a potential auxiliary diagnostic method in the ICU.
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