ArticleStem cell research & therapy2025
Macrophages generated in 3D mechanical microenvironment contribute to recruiting Peritonitis-associated neutrophils in vivo.
Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroudThe influence of scaffolds with bone marrow (BM) niche-like mechanical properties on the stemness and lineage differentiation of hematopoietic stem cells (HSCs) in vitro has been studied. Previous research has demonstrated that 3D collagen hydrogels can regulate the differentiation trajectory of late myeloid progenitors, leading to the specialization of '3D-macrophages' that express various chemokine genes, including Cxcl2 and Cd14. Comprehensive transcriptional profiles in single-cell level have characterized the interaction between 3D-macrophages and neutrophil clusters through the CXCL2/CXCR2 ligand-receptor binding. Therefore, in this study, we aim to confirm the recruited effect of 3D-macrophage subsets on neutrophils during the immune process.
methodsIn this study, 3D collagen hydrogels were constructed to culture HSCs in vitro, then CD14
resultsIn vitro experiments show that 3D-macrophages can recruit neutrophils by expressing Cxcl2. The effect of recruitment can also be observed in the peritonitis mice model, where macrophage ablation is experienced, followed by 3D-macrophage transplantation. CD14
conclusionThese results explored the possible immune function of 3D-macrophages derived from 3D gels, assisting in comprehensively understand immune cell interaction in bone marrow hematopoietic microenvironment. Furthermore, 3D-macrophages are expected to serve as a potential cell therapy for immune deficiency caused by the functional deficiency of macrophages.
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