ArticleBMC research notes2025
Production of functional recombinant antibodies in Dictyostelium discoideum.
Article in BMC research notes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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2 authors.
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Abstract
objectiveRecombinant antibodies are essential reagents for diagnostics, research, and therapy. Numerous production methods have been developed, each of them with its strengths and weaknesses. In this study we evaluated the ability of Dictyostelium discoideum cells to produce and secrete functional antibodies.
resultsThree recombinant antibodies targeting tubulin, CISD1 or CD8β proteins, respectively, were successfully produced and secreted by D. discoideum cells. Electrophoretic analysis of these antibodies revealed a degradation product, resulting from proteolytic cleavage at the linker peptide connecting the scFv portion to the Fc fragment. Removal of this linker suppressed the proteolytic cleavage. Finally, immunofluorescence analysis confirmed that all three antibodies recognized their target antigen in a specific manner. This study represents the first demonstration that functional recombinant antibodies can be produced in D. discoideum cells.
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