ArticleBMC plant biology2025
MmPHR1 enhances low phosphorus stress tolerance by activating MmPHT1;5 in an elite apple rootstock -Malus mandshurica.
Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Soil to Cytoplasm: The Mycorrhizal Phosphorus Express and Its Regulatory Steps.Journal of fungi (Basel, Switzerland) · 2026Review
- The digital orchard: advanced data-driven technologies in apple breeding and genetic modification.Frontiers in plant science · 2025Review
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Authors and funding
6 authors.
Funding
Abstract
backgroundPhosphorus (Pi) is crucial for the growth and development of plants, and Phosphate transporter 1 (PHT1) genes are key in Pi absorption and signaling. PHT1 and phosphate starvation response 1 (PHR1) form the PHR1-PHT1 module, which is essential for Pi uptake and signaling across various species. However, its role in the apple rootstock has not yet been clear so far.
resultsTo gain a clearer insight into the PHR1-PHT1 module's function in coping with low-Pi stress. A transcriptomic analysis of Malus mandshurica, a line with high Pi deficiency tolerance, identified 13 genes of MmPHT1 in response to low-Pi stress. The expression levels of MmPHT1 genes varied in response to low-Pi stress, as shown by qRT-PCR analysis. The analysis of promoter activity revealed that the MmPHT1;5 promoter activated GUS gene expression. The combination of dual-luciferase reporter and yeast one-hybrid (Y1H) assays demonstrated that MmPHR1 interacts with the MmPHT1;5 promoter. Using CRISPR/Cas9 and overexpressing vectors system to transform apple callus to investigate the role of MmPHT1;5 under Pi deficiency. Notably, the OE-MmPHT1;5 line calli showed marked enhancements in acid phosphatase (ACP) activities and total and inorganic Pi contents compared to the wild type. Conversely, the KO-MmPHT1;5 calli showed significant reductions in Pi accumulation, implying it could be involved in the absorption of Pi in apples.
conclusionsMmPHT1;5 plays a substantial role in enhancing low-Pi stress by facilitating Pi absorption in M. mandshurica.
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