Evidence map›Paper›PMID 40467919›Full record

ArticleWorld journal of microbiology & biotechnology2025

Sustainable exploitation of high-protein feather waste for green production of cold-adapted and detergent-stable keratinase by Penicillium oxalicum AUMC 15084.

Ayat M A Abdel-Latif, Nageh F Abo-Dahab, Ahmed M Moharram, Abdallah M A Hassane, Osama A M Al-Bedak

Abstract read
In one paragraph

Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ayat M A Abdel-LatifDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut, 71524, Egypt.
Nageh F Abo-DahabDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut, 71524, Egypt.ORCID http://orcid.org/0000-0002-4793-8528
Ahmed M MoharramDepartment of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, 71511, Egypt.ORCID http://orcid.org/0000-0001-8117-8756
Abdallah M A HassaneDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut, 71524, Egypt. abdallahhassane@azhar.edu.eg.
Osama A M Al-BedakAssiut University Mycological Centre, Assiut, 71511, Egypt. osamaalbedak@science.au.edu.eg.ORCID http://orcid.org/0000-0003-0465-619X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Limited research has investigated the ability of psychrophilic and psychro-tolerant microorganisms to produce cold-active keratinases, despite their potential as an efficient alternative for substrate conversion at reduced energy expenditure. A screening of 32 Penicillium and Talaromyces isolates for keratinolytic activity at temperatures of 5, 10, and 15ºC identified a promising P. oxalicum strain as the most potent at 10ºC, yielding 242.39 U/mL. Following six days of incubation at pH 8.0 and 15 °C with 0.2% yeast extract as the nitrogen source, the P. oxalicum strain exhibited keratinase activity of 359.42 U/mL. The keratinase underwent purification with a 4.13-fold increase, utilizing an MP 800 anion exchanger and Sephacryl S 200 , resulting in a specific activity of 684.46 U/mg and a yield of 5.34%. The SDS-PAGE analysis identified a keratinase with a molecular weight of 37.51 kD, exhibiting peak activity at pH 9.0 and 20ºC, with a specific activity of 721.8 U/mg. Mg

Indexed as

FeathersFungal ProteinsPenicilliumPeptide HydrolasesAnimalsCold TemperatureDetergentsEnzyme StabilityHydrogen-Ion ConcentrationKeratinsMolecular WeightTalaromycesDetergentsFungal ProteinskeratinaseKeratinsPeptide HydrolasesCold-adaptedKeratinaseOptimizationPenicilliumPurification

Identifiers

PMID40467919
PMCPMC12137471

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.