Evidence map›Paper›PMID 40467847›Full record

ArticleCommunications biology2025

Structural insights from vaccine candidates for EV-D68.

Jiaxuan Cheng, Peter W Krug, Haotian Lei, Daniel L Moss, Zabrina C Lang, Abraham J Morton, Chen-Hsiang Shen, Sergei Pletnev, Rick K Huang, Theodore C Pierson and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Open questions in enterovirus uncoating.Journal of virology · 2026
    Review
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Jiaxuan ChengVaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD, 20892, USA.
Peter W KrugVaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD, 20892, USA.ORCID http://orcid.org/0000-0002-5969-1470
Haotian LeiResearch Technologies Branch, NIAID, National Institutes of Health, Bethesda, MD, 20892, USA.ORCID http://orcid.org/0000-0002-4882-0249
Daniel L MossVaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD, 20892, USA.ORCID http://orcid.org/0000-0001-7639-7276
Zabrina C LangLaboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Abraham J MortonLaboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.ORCID http://orcid.org/0009-0006-7278-6227
Chen-Hsiang ShenVaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD, 20892, USA.
Sergei PletnevVaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD, 20892, USA.
Rick K HuangLaboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Theodore C PiersonVaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD, 20892, USA.
Tongqing ZhouVaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD, 20892, USA. tzhou@nih.gov.ORCID http://orcid.org/0000-0002-3935-4637
Tracy J RuckwardtVaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD, 20892, USA. truckwardt@mail.nih.gov.ORCID http://orcid.org/0000-0002-0007-7169
Peter D KwongVaccine Research Center, NIAID, National Institutes of Health, Bethesda, MD, 20892, USA. pdk3@cumc.columbia.edu.ORCID http://orcid.org/0000-0003-3560-232X

Funding

Mechanisms Of Humoral EvasionZ01AI005023 · NIAID · NIAID EXTRAMURAL ACTIVITIES · PI KWONG, PETER D · 2002 to 2008
$564k
Division of Intramural Research, National Institute of Allergy and Infectious Diseases (Division of Intramural Research of the NIAID) AI-005023-23Intramural NIH HHS Z01 AI005023
6 · The paper itself

Abstract

Enterovirus D68 (EV-D68), a member of the Picornaviridae family, causes respiratory illness and can lead to acute flaccid myelitis in children. No specific treatment or vaccine is available. Here, we determine cryo-EM structures of EV-D68 virus-like particles (VLPs), inactivated virus particles (InVPs), and altered virus particles (A-particles) from B3 and A2 subclades. The B3 VLP is a current vaccine candidate, which we show closely resembles its InVP counterpart, particularly at the 5-fold axis of symmetry, the target of potent neutralizing antibodies. Similar structural conservation was observed in the A2 subclade. Sequence variation between B3 and A2 mainly occurred in flexible loops displayed on the particle surface. A canyon-filling pocket factor was present in B3 InVP but absent in A2 InVP. A-particles were predominant in β-propiolactone-inactivated virus at longer but not shorter incubation. Overall, our findings highlight EV-D68 similarities and subclade-specific differences, offering structural insights that relate to vaccine development.

Indexed as

Enterovirus D, HumanEnterovirus InfectionsVaccines, Virus-Like ParticleViral VaccinesAntibodies, NeutralizingCryoelectron MicroscopyHumansModels, MolecularAntibodies, NeutralizingVaccines, Virus-Like ParticleViral Vaccines

Identifiers

PMID40467847
PMCPMC12137623

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.