Evidence map›Paper›PMID 40467842›Full record

ArticleScientific reports2025

Intratracheal instillation of RSV is superior to intranasal inoculation as a model method inducing critical immune cell infiltration.

Yinglin Lin, Yao Xiao, Bin Peng, Mengyuan Xie, Zesen Mai, Mingzhen Lin, Shanshan Miao, Xinru Yang, Wei Li, Yi Wang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yinglin Lin *Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Yao Xiao *Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Bin Peng *Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Mengyuan Xie *Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Zesen MaiKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Mingzhen LinKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Shanshan MiaoKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Xinru YangKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Wei LiKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Yi WangDongguan Key Laboratory for Zoonitic Diseases, Dongguan Bioshine Technology Company, Dongguan City, China.
Zhaoyu LiuKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China. gywyer@gzhmu.edu.cn.
Wenxia YaoKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China. yaowenxia917@gzhmu.edu.cn.

Funding

Guangzhou Key Laboratory of Biological Targeting Diagnosis and Therapy 202201020379he Science and Technology Program of Guangzhou 202102010128Key Laboratory of Guangdong Higher Education Institutes 2021KSYS009Open Project of State Key Laboratory of Respiratory Disease SKLRD-OP-202323the Science and Technology Program of Guangzhou 2024A04J8217
6 · The paper itself

Abstract

Respiratory syncytial virus (RSV) causes a substantial global disease burden, particularly in children and older adults. Despite the availability of three vaccines and two prophylactic monoclonal antibodies, there remains an urgent unmet need for effective RSV treatments. Appropriate experimental animal models and modeling methods are essential for studying RSV pathogenesis and developing therapeutic and preventive agents. In this study, we introduced intratracheal instillation (ITS) method into mouse model of RSV infection and compared it with the conventional intranasal inoculation (INO) method; data showed that ITS method resulted in more efficient viral replication and more severe pathological changes than INO method. Then RNA-seq technology was used to sequence the lung tissues of RSV infected mice with ITS method, and further immunoinfiltration analysis based on transcriptomic profile demonstrated that the ITS approach can lead to the infiltration of multiple innate and adaptive immune cells. It is concluded that ITS of RSV is superior to INO as a model method in mice and can induce infiltration of key immune cells. Collectively, our study offers novel insights into the rational design of future in vivo infection methods with RSV, as well as providing a foundation for the investigation of the immune pathogenesis of RSV.

Indexed as

Respiratory Syncytial VirusesRespiratory Syncytial Virus, HumanRespiratory Syncytial Virus InfectionsAdministration, IntranasalAnimalsDisease Models, AnimalFemaleHumansLungMiceMice, Inbred BALB CVirus ReplicationImmune infiltrationIntratracheal instillationModeling methodRSV

Identifiers

PMID40467842
PMCPMC12137611

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.