ArticleMethods in molecular biology (Clifton, N.J.)2025
A Simplified and Robust Immunofluorescence Labeling Method for Complex 3D Cell Cultures: Minimizing Manipulation and Maximizing Data in Whole-Mount Analysis of Organoids, Spheroids, and Co-culture Models.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whole-mount imaging and labeling of 3D organoids and spheroids offer unparalleled insights into spatial biology, organogenesis, disease mechanisms, and cancer cell dynamics within a physiologically relevant 3D context. Those techniques in biomedical research, yet its application to complex 3D cell culture models, remain fraught with challenges. Current methods often suffer from sample damage and loss, in addition to lengthy and complex protocols involving multiple steps and reagent preparations. To address these challenges, we describe a novel approach that overcomes limitations of conventional techniques by preserving sample integrity, minimizing sample manipulation, and eliminating the need for multiple reagents. The current method facilitates comprehensive sample analysis, significantly improving the efficiency and accuracy of protein visualization. It is compatible with a wide range of samples, including organoids and spheroids in hydrogels, organ-on-chip models, and co-culture systems.
Indexed as
Identifiers
40465186What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.