Evidence map›Paper›PMID 40464976›Full record

ReviewPlanta2025

CRISPR/Cas9: efficient and emerging scope for Brassica crop improvement.

Shiv Shankar Sharma, Ashwani Pandey, Anamika Kashyap, Lakshay Goyal, Pooja Garg, Ranjeet Kushwaha, Jyoti Sharma, Shikha Tripathi, Sujata Kumari, George Thomas and 6 more

Abstract readReview
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In one paragraph

Review in Planta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Shiv Shankar SharmaICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India.
Ashwani PandeyICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India.
Anamika KashyapICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India.
Lakshay GoyalICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India.
Pooja GargICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India.
Ranjeet KushwahaICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India.
Jyoti SharmaICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India.
Shikha TripathiICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India.
Sujata KumariICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India.
George ThomasDepartment of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, Uttar Pradesh, India.
Malkhey VermaSchool of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India.
Navin C GuptaICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India.
Ashish Kumar GuptaICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India.
Ramcharan BhattacharyaICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India. rcbhattacharya1@gmail.com.ORCID http://orcid.org/0000-0001-6274-0643
Sandhya SharmaICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India. biochemsandhya@gmail.com.ORCID http://orcid.org/0000-0002-0518-289X
Mahesh RaoICAR- National Institute for Plant Biotechnology, Pusa campus, New Delhi, 110012, India. mraoicar@gmail.com.ORCID http://orcid.org/0000-0003-3097-292X

Funding

ICAR - National Agricultural Science Fund NASF/BGAM-9021/2022-23/NIPBIndian Council of Agricultural Research 2001-2019
6 · The paper itself

Abstract

MAIN

conclusionCRISPR/Cas9 revolutionizes Brassica crop improvement by enhancing yield, quality, and stress resistance, providing a precise and versatile tool for genetic and agronomic advancements. The rapidly advancing CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) technologies are being employed in both diploid and polyploid species of Brassica for gene functions and precise genetic improvements. CRISPR/Cas technology has sparked significant attention among the scientific community due to its affordability, precision, and effectiveness compared to other genome editing techniques. The recent discoveries highlight the diverse applications of the CRISPR/Cas9 genome editing tool in enhancing agriculturally important traits in Brassica species. This technology has been utilized to improve yield, quality, and resistance to both biotic and abiotic stresses globally. Here, we present an overview that encourages researchers to explore and improve the functionality and genetic progress of Brassica U-triangle species utilizing genome editing technologies. In addition, ethical considerations and concerns associated with CRISPR technologies are addressed, providing valuable insight into how CRISPR/Cas9 tools and have revolutionized crop improvement with special emphasis on Brassica for various agronomically and nutritionally important traits.

Indexed as

BrassicaCRISPR-Cas SystemsCrops, AgriculturalGene EditingGenome, PlantPlant BreedingPlants, Genetically ModifiedAgricultural biotechnologyBrassicaCRISPR/Cas9Genetic improvementGenome editingPolyploid species

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.