Evidence map›Paper›PMID 40464928›Full record

ReviewPlanta2025

A comprehensive review on in vitro therapies for virus elimination and novel methods for virus protection in key horticultural crops.

Karuppasamy Thanuja, Rajangam Arulmozhiyan, Marimuthu Somasundaram Saraswathi, Ramasamy Selvarajan, Vellaichamy Jegadeeswari, Venugopal Rajanbabu

Abstract readReview
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In one paragraph

Review in Planta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Karuppasamy ThanujaDepartment of Fruit Science, Horticultural College and Research Institute for Women, Tiruchirappalli, 620027, Tamil Nadu, India.ORCID http://orcid.org/0009-0001-2548-641X
Rajangam ArulmozhiyanDepartment of Fruit Science, Horticultural College and Research Institute for Women, Tiruchirappalli, 620027, Tamil Nadu, India.ORCID http://orcid.org/0000-0002-9754-1334
Marimuthu Somasundaram SaraswathiICAR - National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli, 620102, Tamil Nadu, India. saraswathimse@gmail.com.ORCID http://orcid.org/0000-0002-4855-4032
Ramasamy SelvarajanICAR - National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli, 620102, Tamil Nadu, India.ORCID http://orcid.org/0000-0002-9766-3147
Vellaichamy JegadeeswariDepartment of Fruit Science, Horticultural College and Research Institute for Women, Tiruchirappalli, 620027, Tamil Nadu, India.ORCID http://orcid.org/0000-0002-8345-0570
Venugopal RajanbabuDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India.ORCID http://orcid.org/0000-0001-8761-3333

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MAIN

conclusionThis review examines the impact of viable in vitro methods and novel strategies on virus elimination in key horticultural crops. Virus diseases are a major threat to economically important horticultural crops, leading to severe financial losses for farmers due to the lack of effective management strategies. Therefore, there is an urgent need for disseminating comprehensive knowledge on viable strategies available to mitigate adverse impacts. This review provides an overview of the effectiveness of conventional in vitro techniques on virus elimination, including meristem culture, chemotherapy, thermotherapy, cryotherapy, electrotherapy, and their combinations. It also explores the significance of innovative methods for the development of transgenic varieties through gene transformation, CRISPR-Cas9 system and RNA interference techniques. Conventional techniques can effectively utilize virus-infected plant material for the production of virus-free plantlets, while novel approaches include the use of biotechnological tools for the development of resistant cultivars. It outlines the results obtained from the application of various in vitro methods in different horticultural crops. This also highlights the factors determining the success rate of various therapies such as meristem size, antiviral chemical concentrations, optimal temperature requirements, effective cryotherapy protocols and the necessary electrical supply for viral strains across various cultivars of crop plants. The virus elimination rates of these in vitro approaches ranged from 50 to 100%, depending on the specific virus, host crop involved, and the technique applied. Additionally, the review examines the development of transgenic resistant cultivars in response to specific viruses infecting important crops. The summarized results on virus elimination offer valuable insights about the strategies used to counteract the emerging viral strains that threaten the integrity of horticultural crop production.

Indexed as

Crops, AgriculturalPlant DiseasesPlant VirusesPlants, Genetically ModifiedChemotherapyCryotherapyElectrotherapyHorticultural cropsMeristem cultureThermotherapyVirusVirus elimination

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.