ArticleNucleic acids research2025
In vivo single-molecule imaging of RecB reveals efficient repair of DNA damage in Escherichia coli.
Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- The B. subtilis translesion polymerase Pol Y1 is not strongly recruited to sites of replication upon different types of DNA damage.PLoS genetics · 2026Article
- The RecBCD complex interacts directly with the DNA sliding clamp in Escherichia coli.Nucleic acids research · 2026Article
- "ThebioRxiv : the preprint server for biology · 2026Article
- Daily life in the Open Biologist's second job, as a Data Curator.Wellcome open research · 2024Article
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8 authors.
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Abstract
Efficient DNA repair is essential for maintaining genome integrity and ensuring cell survival. In Escherichia coli, RecBCD plays a crucial role in processing DNA ends, following a DNA double-strand break (DSB), to initiate repair. While RecBCD has been extensively studied in vitro, less is known about how it contributes to rapid and efficient repair in living bacteria. Here, we use single-molecule microscopy to investigate DNA repair in real time in E. coli. We quantify RecB single-molecule mobility and monitor the induction of the DNA damage response (SOS response) in individual cells. We show that RecB binding to DNA ends caused by endogenous processes leads to efficient repair without SOS induction. In contrast, repair is less efficient in the presence of exogenous damage or in a mutant strain with modified RecB activities, leading to high SOS induction. Our findings reveal how subtle alterations in RecB activity profoundly impact the efficiency of DNA repair in E. coli.
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