ArticleFrontiers in immunology2025
Single-cell transcriptomics in colorectal cancer uncover the potential of metastasis and immune dysregulation of a cell cluster overexpressed PRSS22.
Article in Frontiers in immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Article
- PRSS22 as a novel prognostic and immune regulatory biomarker in PanCancer multiomics analysis.Scientific reports · 2025Article
- Machine learning identifies PYGM as a macrophage polarization-linked metabolic biomarker in rectal cancer prognosis.Frontiers in immunology · 2025Article
- Combined serine protease PRSS22 and CEA mRNA analysis identifies the majority of colon cancer patients that recur within 12 years.Frontiers in oncology · 2025Article
- PRSS22 promotes the immune evasion of gastric cancer via inhibiting ANXA1-mediated degradation of PD-1.Food & nutrition research · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
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Abstract
Background: Colorectal cancer (CRC) is one of the most common malignancies worldwide, and its complex pathogenesis and significant tumor cell heterogeneity remain major challenges. With the rapid development of single-cell sequencing technology, we can now delve deeper into the cellular composition and dynamic changes within the tumor microenvironment, revealing cellular interactions and their potential roles in tumorigenesis. Method: In this study, we systematically analyzed comprehensive single-cell RNA sequencing data from 25 colorectal cancer and 10 adjacent normal tissue samples. We explored the characteristics and biological significance of tumor cell subpopulations, performed quality control, dimensionality reduction, and cell type identification, and further investigated epithelial cell copy number variations, cell communication, and pseudotime analysis. Subsequently, Boruta feature selection algorithm was combined to identify prognosis related genes. The expression patterns, clinical significance and biological effects of Results: Our analysis found an epithelial cell subcluster with high expression of Conclusion: Our study has identified a cell subcluster that is closely linked to progression, immune dysregulation and prognosis in CRC, and we have also identified
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