Evidence map›Paper›PMID 40462940›Full record

ArticlebioRxiv : the preprint server for biology2025

Two-Dimensional Nonlinear Structured Illumination Microscopy with rsEGFP2.

Shaoheng Li, Ryo Tamura, Kota Banzai, Daichi Kamiyama, Peter Kner

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Shaoheng LiSchool of Electrical and Computer Engineering, University of Georgia, Athens, GA, USA.
Ryo TamuraDepartment of Cellular Biology, University of Georgia, Athens, GA, USA.
Kota BanzaiDepartment of Cellular Biology, University of Georgia, Athens, GA, USA.
Daichi KamiyamaDepartment of Cellular Biology, University of Georgia, Athens, GA, USA.ORCID 0000-0002-1466-5295
Peter KnerSchool of Electrical and Computer Engineering, University of Georgia, Athens, GA, USA.ORCID 0000-0001-7555-5180

Funding

Three-dimensional Nonlinear Structured Illumination for Live Imaging with 80 nm resolutionR01GM149978 · NIGMS · UNIVERSITY OF GEORGIA · PI Peter Alexander Kner · 2023 to 2026
$1.2M
Screening using split fluorescent protein tags for neurotransmitter receptors that define a synaptic balance in neuralcircuitsR21NS128750 · NINDS · UNIVERSITY OF GEORGIA · PI KAMIYAMA, DAICHI · 2023 to 2023
$404k
NIGMS NIH HHS R01 GM149978NINDS NIH HHS R21 NS128750
6 · The paper itself

Abstract

Superresolution microscopy enables imaging of subcellular structures and dynamics with nanoscale detail. Among the various superresolution techniques, structured illumination microscopy (SIM) stands out for its compatibility with live-cell imaging. Linear SIM is restricted to a resolution improvement of a factor of two, improving the resolution to about 100 nm. Nonlinear SIM (NSIM) utilizes reversibly switchable fluorescent proteins to generate a nonlinear response, allowing for the collection of higher spatial frequency information and theoretically extending the resolution without limit. By employing rsEGFP2 and patterned depletion illumination (PD) to generate the desired nonlinearity in the fluorescent response, we have successfully achieved 2D PD-NSIM imaging of actin in live U2OS cells with sub-80 nm resolution.

Identifiers

PMID40462940
PMCPMC12132326

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.