ArticlebioRxiv : the preprint server for biology2025
New gene annotations for three pea aphid genome assemblies allow comparative analyses of genes and gene family evolution.
Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Reliable genome annotation is crucial for analyses of gene function, conservation, and evolution. Factors such as the sequencing technology used to create the assembly and the amount of duplicated sequence within the genome of interest can have a large impact on the quality of gene annotations. In particular, short read-based assemblies tend to mis-assemble duplicated genes as single loci, a problem that requires additional long read sequencing to resolve. Pea aphids exhibit a high level of gene duplication, resulting in mis-assembly and mis-annotation of genes in the short read reference genome. Here, we re-annotate the pea aphid reference genome, along with two long read pea aphid genomes, to facilitate future analyses of gene duplication and function in pea aphids. We use an integrated approach, consolidating both
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