ArticleStem cell research & therapy2025
Sea buckthorn-derived extracellular vesicles foster bone regeneration through aau-miR168-mediated pathways.
Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Plant-derived extracellular vesicles as bioactive nanoplatforms: Biogenesis, therapeutic evidence, and translational challenges.Biochemistry and biophysics reports · 2026Review
- Triple identity of medicinal plant-derived extracellular vesicles.Protoplasma · 2026Review
- Apple-Derived Vesicles Orchestrate Bone Regeneration:International journal of molecular sciences · 2026Article
- The recent progression of extracellular vesicles application in osteoporosis.Frontiers in pharmacology · 2026Review
- Chinese herbal medicine-derived exosome-like nanovesicles for orthopaedic diseases: evidence, methods, and translation.Frontiers in cell and developmental biology · 2026Review
- Research Progress and Preclinical Prospects of Plant-Derived Extracellular Vesicles in Targeted Delivery of Antitumor Drugs.International journal of nanomedicine · 2026Review
- Immunocyte-derived extracellular vesicles in osteoimmunology: mechanisms, disease contexts, and translational prospects.Frontiers in immunology · 2025Review
- Extracellular vesicles in reproductive medicine: from "animal-led" to "plant-enabled".Frontiers in cell and developmental biology · 2025Review
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12 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundPlant-derived extracellular vesicles (P-EVs) possess remarkable therapeutic potential, yet the regenerative capabilities of sea buckthorn-derived extracellular vesicles (SAEVs) remain underexplored. This study aims to elucidate the osteogenic and bone-healing properties of SAEVs.
methodsSAEVs were isolated from sea buckthorn juice via differential centrifugation and characterized using electron microscopy and dynamic light scattering. Bone marrow mesenchymal stromal cells (BMSCs) were treated with SAEVs, and cellular uptake was evaluated through fluorescence microscopy and flow cytometry. In vivo, DiD-labeled SAEVs were orally administered to mice to determine biodistribution using IVIS imaging. A murine femoral defect model was employed to assess the bone regenerative efficacy of SAEVs delivered with or without GelMA hydrogels, analyzed by micro-CT and histological staining. Small RNA sequencing identified SAEV-derived miRNAs, and luciferase reporter assays validated the miRNA-mediated regulation of osteogenic genes.
resultsSAEVs efficiently internalized into BMSCs via macropinocytosis, promoting the expression of key osteogenic markers such as Runx2 and osteocalcin. In vivo, SAEV-GelMA hydrogels significantly accelerated bone regeneration in a femoral defect model without inducing adverse hematological effects, affirming the safety of SAEV administration. Mechanistic investigations revealed an enrichment of miRNAs, particularly aau-miR168, which modulates osteogenesis through the aau-miR168/LBH/RUNX2 signaling cascade.
conclusionsThis study highlights SAEVs as a transformative and biocompatible therapeutic strategy for fracture healing and osteoporosis management, offering a novel avenue for regenerative medicine.
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