ArticleClinical proteomics2025
Plasma exosome proteomics reveals upregulation of CILP-1 in concave side of paraspinal muscle in adolescent idiopathic scoliosis.
Article in Clinical proteomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- CT-Derived Paraspinal Muscle Asymmetry Is Associated with Deformity Severity in Adolescent Idiopathic Scoliosis: A Quantitative CT Study.Journal of clinical medicine · 2026Article
- Pathogenic mechanisms of adult degenerative scoliosis.Frontiers in cell and developmental biology · 2026Review
- Current insights into circulating biomarkers and their potential for predicting adolescent idiopathic scoliosis progression.Frontiers in cell and developmental biology · 2026Review
- Asymmetrical paravertebral muscles fibrosis causes progression of adolescent idiopathic scoliosis via myostatin signalling in fibro-adipogenic progenitors.Journal of orthopaedic translation · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThere is insufficient attention to the pathogenesis of abnormal radiological changes and molecular mechanism in the paraspinal muscles in AIS patients.
methodsProteomics of plasma exosomes were applied for identification of differentially expressed proteins (DEPs) in AIS patients through liquid chromatography mass spectrometry (LC-MS/MS). Bioinformatic analysis were performed to explore biomarkers. The muscle density (HU value) of the concave and convex sides of paravertebral muscles in AIS patients was compared. HE staining were applied for investigation of pathological changes of paravertebral muscles. Cartilage intermediate layer protein-1 (CILP-1), TGF-β1/Smad pathway and the downstream proteins were compared between the concave and convex side of paraspinal muscle. C2C12 cells were incubated with TGF-β1 or Smad3 phosphorylation inhibitor (SIS3) to further clarify the correlation between CILP-1 and TGF-β1/Smad pathway.
resultsA total of 2437 proteins were identified, among which DEPs were enriched in immune response and extracellular matrix-receptor interaction, while CILP-1 was screened out. HU value of concave multifidus muscle (MF) in apical vertebrae area was significantly lower when compared with both convex MF and control group. Muscle fibrosis, increased CILP-1, TGF-β1 phosphorylation of Smad2/3 and downstream proteins could be observed in the concave side of paraspinal muscle. TGF-β1 stimulation resulted in upregulation of CILP-1 and ECM related proteins, which could be partially inhibited by SIS3. DISCUSSION: We confirmed the asymmetric expressions of CILP-1 and TGF-β1/Smad signaling pathways in the paravertebral muscles of AIS patients. In C2C12 cells, TGF-β1 induced up-regulation of CILP-1 expression via Smad3 phosphorylation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.