ArticleMolecular biology reports2025
Transcriptional regulation of solute carrier family 6 member 9 gene.
Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Characterization of Novel Decenoic Acid Derivatives Exhibiting Cytotoxic Activity Against T98G Glioma Cells Under a Serum- and Glucose-deprived Condition.Cell biochemistry and biophysics · 2026Article
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Authors and funding
5 authors.
Funding
Abstract
backgroundSince the endoplasmic reticulum (ER) stress response is found in almost all tissues, its regulation and downstream factors have been vigorously explored. METHODS AND
resultsIn this study, we performed a comprehensive microarray analysis of genes induced by tunicamycin (Tm) stimulation in the rat cardiac fibroblast cell line H9c2. Tm treatment induced a marked increase in solute carrier family 6 member 9, Slc6a9, mRNA as well as typical ER stress-inducible factors GADD153 and GRP78 mRNA. Slc6a9 mRNA in H9c2 cells was also increased by ER stress inducers thapsigargin (Tg) and brefeldin A (BFA) other than Tm. The effects of inhibitors on the ER-localized stress sensors IRE1, ATF6, and PERK were examined, and the PERK inhibitor GSK2606414 significantly suppressed Tm-induced Slc6a9 mRNA expression. Analysis of Slc6a9 mRNA expression in PERK and ATF4-deficient HEK293 cells established by genome editing showed that Slc6a9 mRNA expression by Tm was suppressed in these cells. Furthermore, the long variant of Slc6a9 mRNA was increased in Tm-treated HEK293, while the short variant was negligible. Analysis of the nucleotide sequence of the 5'-flanking region of the Slc6a9 gene revealed an ATF4-binding sequence in exon1 is highly conserved among many species. As expected, our luciferase reporter assay containing the ATF4 binding sequence near the transcription start site showed a marked increase in promoter activity upon Tm stimulation and ATF4 co-expression.
conclusionsThe ATF4-binding sequence found near the transcription start site of the Slc6a9 gene is highly conserved among species and the element is functional in the PERK-ATF4 system. This study will help elucidate Slc6a9-mediated regulation of intracellular and extracellular amino acid homeostasis under pathophysiological conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.