Evidence map›Paper›PMID 40461574›Full record

ArticleNature communications2025

H3.3 deposition counteracts the replication-dependent enrichment of H3.1 at chromocenters in embryonic stem cells.

Stefano Arfè, Tina Karagyozova, Audrey Forest, Dominic Bingham, Hatem Hmidan, David Mazaud, Mickaël Garnier, Patricia Le Baccon, Eran Meshorer, Jean-Pierre Quivy and 1 more

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Stefano ArfèNuclear Dynamics, Institut Curie, PSL University, Sorbonne Université, CNRS, Paris, France.ORCID http://orcid.org/0000-0002-1514-6366
Tina KaragyozovaNuclear Dynamics, Institut Curie, PSL University, Sorbonne Université, CNRS, Paris, France.ORCID http://orcid.org/0000-0002-5923-5678
Audrey ForestNuclear Dynamics, Institut Curie, PSL University, Sorbonne Université, CNRS, Paris, France.
Dominic BinghamNuclear Dynamics, Institut Curie, PSL University, Sorbonne Université, CNRS, Paris, France.ORCID http://orcid.org/0009-0001-8831-5175
Hatem HmidanNuclear Dynamics, Institut Curie, PSL University, Sorbonne Université, CNRS, Paris, France.
David MazaudNuclear Dynamics, Institut Curie, PSL University, Sorbonne Université, CNRS, Paris, France.ORCID http://orcid.org/0000-0003-3998-9330
Mickaël GarnierNuclear Dynamics, Institut Curie, PSL University, Sorbonne Université, CNRS, Paris, France.ORCID http://orcid.org/0000-0002-3999-0879
Patricia Le BacconNuclear Dynamics, Institut Curie, PSL University, Sorbonne Université, CNRS, Paris, France.ORCID http://orcid.org/0000-0001-8650-4995
Eran MeshorerDepartment of Genetics, The Alexander Silberman Institute for Life Science, and the Edmond and Lily Safra Center for Brain Sciences (ELSC), The Hebrew University of Jerusalem, Jerusalem, Israel.ORCID http://orcid.org/0000-0003-4777-986X
Jean-Pierre QuivyNuclear Dynamics, Institut Curie, PSL University, Sorbonne Université, CNRS, Paris, France. jean-pierre.quivy@curie.fr.ORCID http://orcid.org/0000-0001-6557-7204
Geneviève AlmouzniNuclear Dynamics, Institut Curie, PSL University, Sorbonne Université, CNRS, Paris, France. Genevieve.almouzni@curie.fr.ORCID http://orcid.org/0000-0001-5570-0723

Funding

Agence Nationale de la Recherche (French National Research Agency) ANR-10-IDEX-0001-02Agence Nationale de la Recherche (French National Research Agency) ANR-11LABX-0044_DEEPLigue Contre le Cancer Labellisation
6 · The paper itself

Abstract

Chromocenters in mouse cells are membrane-less nuclear compartments representing typical heterochromatin stably maintained during cell cycle. We explore how histone H3 variants, replicative H3.1/2 or replacement H3.3, mark these domains during the cell cycle in mouse embryonic stem cells, neuronal precursor cells as well as immortalized 3T3 cells. We find a strong and distinct H3.1 enrichment at chromocenters, with variation in mouse embryonic stem cells. Mechanistically, this H3.1 selective enrichment depends on the DNA Synthesis Coupled deposition pathway operating in S phase challenged when we target H3.3 deposition through the DNA Synthesis Independent deposition pathway mediated by HIRA. Altering the H3.1/H3.3 dynamics at chromocenters in mouse embryonic stem cells affects nuclear morphology and cell division. Here, we reveal opposing mechanisms for H3.1 and H3.3 deposition with different enforcement according to cell cycle and potency which determine their ratio at chromocenters and are critical for genome stability and cell survival.

Indexed as

DNA ReplicationEmbryonic Stem CellsHistonesMouse Embryonic Stem Cells3T3 CellsAnimalsCell CycleCell NucleusHeterochromatinMiceHeterochromatinHistones

Identifiers

PMID40461574
PMCPMC12134209

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.