Evidence map›Paper›PMID 40461424›Full record

ArticleJournal of veterinary science2025

Development of visual dye-based loop-mediated isothermal amplification assays for

Sudu Hakuruge Madusha Pramud Wimalasena, Sang Young Seo, Han Gyu Lee, Young Hun Jung, Yoon Jung Do, Ara Cho, Changyong Choe, Are Sun Yoo, Seogjin Kang, Jun Ho Lee and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. East AsianJournal of veterinary science · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Sudu Hakuruge Madusha Pramud Wimalasena *Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.ORCID https://orcid.org/0000-0002-9896-1592
Sang Young Seo *Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.ORCID https://orcid.org/0000-0002-7823-9295
Han Gyu LeeDivision of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.ORCID https://orcid.org/0000-0002-3531-1971
Young Hun JungDivision of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.ORCID https://orcid.org/0000-0002-8094-0304
Yoon Jung DoDivision of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.ORCID https://orcid.org/0000-0003-3207-3514
Ara ChoDivision of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.ORCID https://orcid.org/0000-0001-5309-7721
Changyong ChoeDivision of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.ORCID https://orcid.org/0000-0003-4222-3360
Are Sun YooDivision of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.ORCID https://orcid.org/0000-0001-7258-2626
Seogjin KangDivision of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.ORCID https://orcid.org/0000-0001-7838-9042
Jun Ho LeeDepartment of Infectious Disease, College of Veterinary Medicine, and BK21, Seoul National University, Seoul 08826, Korea.ORCID https://orcid.org/0009-0008-8953-6413
Han Sang YooDepartment of Infectious Disease, College of Veterinary Medicine, BK21 and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.ORCID https://orcid.org/0000-0003-0548-0835
Eun-Yeong BokDivision of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea. eybok@korea.kr.ORCID https://orcid.org/0000-0002-1045-9670

Funding

National Institute of Animal Science
6 · The paper itself

Abstract

importance

objectiveIn this study, we aimed to develop a new primer set for the loop-mediated isothermal amplification (LAMP) method for facilitating visual-based detection of MAP.

methodsWe designed LAMP primers for the MAP-specific gene IS

resultsThe LAMP method demonstrated sensitivity superior to that of the conventional polymerase chain reaction (PCR) method and equal to that of real-time-PCR targeting the F CONCLUSIONS AND RELEVANCE: The developed mixed dye-LAMP method facilitates immediate, sensitive diagnosis by providing a user-friendly readout. By reducing diagnostic time and enhancing visual clarity without specialized equipment, it significantly improves JD control in the livestock industry.

Indexed as

Cattle DiseasesGoat DiseasesMolecular Diagnostic TechniquesMycobacterium avium subsp. paratuberculosisNucleic Acid Amplification TechniquesParatuberculosisAnimalsCattleFecesGoatsRepublic of KoreaSensitivity and SpecificitydiagnosisIS1311Johne's diseasemixed dye-loop-mediated isothermal amplification

Identifiers

PMID40461424
PMCPMC12146026

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.