Evidence map›Paper›PMID 40461419›Full record

ArticleJournal of veterinary science2025

Determination of tissue tropism of chicken anaemia virus on chicken embryos: a molecular insight.

Sedeneinuo Suohu, Gurusamipalayam Amirthalingam Balasubramaniam, Ayyasamy Arulmozhi, Thippichettipalayam Ramasamy Gopalakrishnamurthy, Angamuthu Raja

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Article in Journal of veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Sedeneinuo SuohuDepartment of Veterinary Pathology, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Namakkal 637 002, India. iamsedesuohu@gmail.com.ORCID https://orcid.org/0009-0007-4918-919X
Gurusamipalayam Amirthalingam BalasubramaniamDepartment of Veterinary Pathology, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Namakkal 637 002, India.ORCID https://orcid.org/0000-0001-7981-6843
Ayyasamy ArulmozhiDepartment of Veterinary Pathology, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Namakkal 637 002, India.ORCID https://orcid.org/0000-0003-2899-8733
Thippichettipalayam Ramasamy GopalakrishnamurthyPoultry Disease Diagnosis and Surveillance Laboratory, Veterinary College and Research Institute Campus, Tamil Nadu Veterinary and Animal Sciences University, Namakkal 637 002, India.ORCID https://orcid.org/0000-0003-4325-496X
Angamuthu RajaDepartment of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai 600 007, India.ORCID https://orcid.org/0000-0002-5477-2730

Funding

Tamil Nadu Veterinary and Animal Sciences University
6 · The paper itself

Abstract

importanceFew studies have examined the interactions of the chicken anemia virus (CAV) with the immune system of embryos.

objectiveTo study the pathogenicity of the CAV in chicken embryos with respect to its tissue tropism and viral load in different tissues.

methodThe pathogenicity of the CAV was examined in about-to-hatch embryos through cytology, histopathological examinations, molecular detection, immunocytochemistry (ICC), immunofluorescent test, and immunohistochemistry techniques.

resultsThe experimental study using a polymerase chain reaction (PCR) assay revealed the presence of CAV in the liver, bone marrow, and thymus tissues of embryos where embryos were inoculated with CAV-positive bone marrow specimens obtained from chicken anemia-suspect cases. Intracytoplasmic and intranuclear inclusion bodies were detected cytologically in the bone marrow of the embryos, whereas no inclusion body was detected in the thymus. On the other hand, both kinds of inclusion bodies were observed in bone marrow and thymus in the histopathology. In addition, various organs of PCR-positive CAV embryos revealed the depletion of lymphocytes and hematopoietic cells in the thymus and bone marrow, respectively. The CAV antigen was confirmed in the bone marrow and thymus cytological smears using ICC and immunofluorescence techniques. CONCLUSIONS AND RELEVANCE: Viral antigen exhibited more tropism towards the bone marrow of embryos than the thymus in ICC and immunofluorescence techniques. The presence of CD44, a marker for progenitor cells and an antigen in the serial sections of bone marrow, confirmed the relevance of viral tropism for the hemocytoblasts of the bone marrow as the main target cells in embryos and subsequently in chicks for viral replication.

Indexed as

Chicken anemia virusCircoviridae InfectionsPoultry DiseasesViral TropismAnimalsBone MarrowChick EmbryoChickensImmunohistochemistryLiverPolymerase Chain ReactionThymus GlandViral LoadAntigens, CD44Chicken anemia virusembryoimmunosuppressionprogenitor cells

Identifiers

PMID40461419
PMCPMC12146021

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