Evidence map›Paper›PMID 40460455›Full record

ArticleJournal of the American Chemical Society2025

Microcrystal Electron Diffraction-Guided Discovery of Fungal Metabolites.

David A Delgadillo, Lin Wu, Caroline Wang, Yalong Zhang, Kunal K Jha, Jessica E Burch, Lygia Silva de Moraes, Isabel Hernandez Rodriguez, Melody J Tang, Gerald F Bills and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Chemical Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Rapid Structural Analysis of Natural Products Using MicroED.Small (Weinheim an der Bergstrasse, Germany) · 2026
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

David A DelgadilloDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.ORCID 0000-0002-0897-4470
Lin WuDepartments of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States.
Caroline WangDepartment of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, United States.
Yalong ZhangDepartments of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States.
Kunal K JhaDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.
Jessica E BurchDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.
Lygia Silva de MoraesDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.
Isabel Hernandez RodriguezDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.
Melody J TangDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.
Gerald F BillsTexas Therapeutic Institute, The Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, Texas 77054, United States.ORCID 0000-0003-2352-8417
Benjamin P TuDepartment of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, United States.ORCID 0000-0001-5545-9183
Yi TangDepartments of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States.ORCID 0000-0003-1597-0141
Hosea M NelsonDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.ORCID 0000-0002-4666-2793

Funding

Development and validation of a high-throughput MicroED-driven platform technology for natural product discoveryR01AT011990 · NCCIH · EMORY UNIVERSITY · PI Hosea Martin Nelson, CASSANDRA LEAH QUAVE · 2022 to 2026
$3.2M
NCCIH NIH HHS R01 AT011990
6 · The paper itself

Abstract

Nature remains a vast repository of complex and functional metabolites whose structural characterization continues to drive innovations in pharmaceuticals, agrochemicals, and materials science. The cryogenic electron microscopy (cryoEM) method, microcrystal electron diffraction (microED, a 3D ED technique), has emerged as a powerful tool to structurally characterize small molecules. Despite this emerging role in structural chemistry, the cost and throughput of microED have limited its application in the discovery of natural products (NPs). While recent advances in sample preparation (e.g., arrayED) have provided a conceptual framework to address these challenges, they have remained unproven. Herein, we report the arrayED-driven discovery of a structurally unprecedented family of NPs (zopalides A-E), a muurolane-type sesquiterpene glycoside (rhytidoside A), aspergillicin analogs (aspergillicins H and I), and four crystal structures of previously reported fungal metabolites. Lastly, this the first time that the absolute stereo configuration of newly discovered NPs has been determined directly by microED alone without other methods using a small amount of sample.

Indexed as

Biological ProductsFungiSesquiterpenesCryoelectron MicroscopyCrystallography, X-RayElectronsModels, MolecularMolecular StructureBiological ProductsSesquiterpenes

Identifiers

PMID40460455
PMCPMC12314910

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.