Evidence map›Paper›PMID 40460257›Full record

ArticleChemistry & biodiversity2025

Anxiolytic Effects of Indole Alkaloids From Rauvolfia ligustrina in Adult Zebrafish: Involvement of the GABAergic and 5-HT Systems.

Nádia Aguiar Portela Pinheiro, Ivana Carneiro Romão, Amanda Maria Barros Alves, Sônia Maria Costa Siqueira, Jane Eire Silva Alencar de Menezes, Emmanuel Silva Marinho, Márcia Machado Marinho, Herbert de Sousa Magalhães, Andreia Ferreira de Castro Gomes, Otília Deusdênia Loiola Pessoa and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemistry & biodiversity, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Nádia Aguiar Portela PinheiroGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Ivana Carneiro RomãoGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Amanda Maria Barros AlvesGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Sônia Maria Costa SiqueiraGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Jane Eire Silva Alencar de MenezesGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Emmanuel Silva MarinhoGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.
Márcia Machado MarinhoCenter for Exact Sciences and Technology, State University of Vale do Acaraú, Sobral, Brazil.
Herbert de Sousa MagalhãesDepartment of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, Brazil.
Andreia Ferreira de Castro GomesDepartment of Biology, Environmental Biology, School of Sciences, University of Minho, Braga, Portugal.
Otília Deusdênia Loiola PessoaDepartment of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, Brazil.
Hélcio Silva Dos SantosGraduate Program in Natural Sciences, State University of Ceará, Fortaleza, Brazil.ORCID https://orcid.org/0000-0001-5527-164X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Benzodiazepines are widely used in the treatment of anxiety, but their use may be interrupted due to side effects. This study evaluated the indole alkaloids isocarapanaubine (indole alkaloid 1 [AIN1]) and 18-β-hydroxy-3-epi-α-yohimbine (indole alkaloid 2 [AIN2]), extracted from Rauvolfia ligustrina, in adult zebrafish. Three doses (4, 12, and 20 mg/kg) were tested, along with dimethyl sulfoxide (DMSO) 3% and Diazepam (4 mg/kg) in toxicity, locomotion, and anxiolytic effect assays. AIN1 and AIN2 showed low toxicity (lethal dose capable of killing 50% of the animals >20 mg/kg) and reduced locomotor activity. Both exhibited anxiolytic effects, with AIN1 acting through the GABAergic system and AIN2 via the serotonergic pathway (5-HT

Indexed as

Anti-Anxiety AgentsIndole AlkaloidsRauwolfiaSerotoninAnimalsDose-Response Relationship, DrugLocomotionMolecular Docking SimulationMolecular StructureReceptors, SerotoninStructure-Activity RelationshipZebrafishAnti-Anxiety AgentsIndole AlkaloidsReceptors, SerotoninSerotoninanxietyDMPKindole alkaloidsmolecular dockingzebrafish

Identifiers

PMID40460257
PMCPMC12533794

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.