Evidence map›Paper›PMID 40459771›Full record

ArticleMedical microbiology and immunology2025

Adaptation and validation of a gastrointestinal panel to detect diarrheal virus pathogens on a high-throughput qPCR system.

Katja Giersch, Dominik Nörz, Moritz Grunwald, Susanne Pfefferle, Lisa Sophie Pflüger, Nicole Fischer, Martin Aepfelbacher, Marc Lütgehetmann

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical microbiology and immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Katja Giersch *Institute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf (UKE), Martinistraße 52, D-20246, Hamburg, Germany.
Dominik Nörz *Institute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf (UKE), Martinistraße 52, D-20246, Hamburg, Germany.
Moritz GrunwaldInstitute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf (UKE), Martinistraße 52, D-20246, Hamburg, Germany.
Susanne PfefferleInstitute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf (UKE), Martinistraße 52, D-20246, Hamburg, Germany.
Lisa Sophie PflügerInstitute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf (UKE), Martinistraße 52, D-20246, Hamburg, Germany.
Nicole FischerInstitute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf (UKE), Martinistraße 52, D-20246, Hamburg, Germany.
Martin AepfelbacherInstitute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf (UKE), Martinistraße 52, D-20246, Hamburg, Germany.
Marc LütgehetmannInstitute of Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf (UKE), Martinistraße 52, D-20246, Hamburg, Germany. mluetgeh@uke.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

With around 2 billion cases each year, infectious gastroenteritis remains a worldwide health problem. A major cause of acute gastroenteritis is infection with enteric viruses, which often leads to hospitalization in children and immunocompromised people. We adapted and validated a gastrointestinal qPCR panel, which simultaneously detects the most common enteric viruses: Norovirus GI and GII, Rotavirus, Adenovirus, Sapovirus, Astrovirus and Enterovirus in stool samples on a fully automated, high-throughput system (Roche cobas5800/6800/8800). Limits of detection (LOD), linear range and precision were determined using dilutions of clinical stool samples, which were quantified by digital droplet PCR. Specificity and sensitivity were evaluated using clinical stool samples from patients with diarrhoea and results were compared with commercial CE-IVD qPCR assays. LODs were below 100 for all targets except for Norovirus GI (3,180 copies/ml), Norovirus GII (299 copies/ml) and Rotavirus (851 copies/ml). The assay showed excellent linearity over 5-6 log steps for all pathogens (r

Indexed as

DiarrheaGastroenteritisHigh-Throughput Screening AssaysMolecular Diagnostic TechniquesReal-Time Polymerase Chain ReactionVirusesFecesHumansLimit of DetectionSensitivity and SpecificityAdenovirusAstrovirusCobas 5800Cobas 6800Cobas 8800EnterovirusInfectionMolecular diagnosticsNorovirusReal time polymerase chain reactionRotavirusSapovirus

Identifiers

PMID40459771
PMCPMC12134015

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.