Evidence map›Paper›PMID 40459708›Full record

ArticleTransgenic research2025

First report of transgenic Agave sisalana 'RLV 19' plants resistant to the herbicide glyphosate-an improved alternative for biofuel production.

Carolina Rossi de Oliveira, Aline Vitória Corim Marim, Camila Santos Woloche, Paulo Cezar De Lucca, Marcelo Falsarella Carazzolle, Gonçalo Amarante Guimarães Pereira

Abstract read
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Article in Transgenic research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Carolina Rossi de OliveiraGenomics and BioEnergy Laboratory, University of Campinas and Biology Institute IB, Rua Carl Von Linneaus, S/N, Cidade Universitária, UNICAMP, Campinas, SP, Brazil.ORCID http://orcid.org/0000-0002-3563-4834
Aline Vitória Corim MarimGenomics and BioEnergy Laboratory, University of Campinas and Biology Institute IB, Rua Carl Von Linneaus, S/N, Cidade Universitária, UNICAMP, Campinas, SP, Brazil.ORCID http://orcid.org/0009-0005-1272-4051
Camila Santos WolocheGenomics and BioEnergy Laboratory, University of Campinas and Biology Institute IB, Rua Carl Von Linneaus, S/N, Cidade Universitária, UNICAMP, Campinas, SP, Brazil.ORCID http://orcid.org/0000-0003-3161-9327
Paulo Cezar De LuccaGenomics and BioEnergy Laboratory, University of Campinas and Biology Institute IB, Rua Carl Von Linneaus, S/N, Cidade Universitária, UNICAMP, Campinas, SP, Brazil.ORCID http://orcid.org/0000-0001-6492-3515
Marcelo Falsarella CarazzolleGenomics and BioEnergy Laboratory, University of Campinas and Biology Institute IB, Rua Carl Von Linneaus, S/N, Cidade Universitária, UNICAMP, Campinas, SP, Brazil.ORCID http://orcid.org/0000-0002-5474-2830
Gonçalo Amarante Guimarães PereiraGenomics and BioEnergy Laboratory, University of Campinas and Biology Institute IB, Campinas, SP, Brazil. goncalo@unicamp.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Agave genus is recognized for its diversity, economic importance, and adaptability to arid and semi-desert climates. Among its species, Agave sisalana stands out for the production of sisal, a resilient natural fiber with various industrial applications and potential to be used as a raw material in biofuel production, due to the accumulation of fermentable sugars in its biomass. Although this species presents significant agronomic potential, challenges in conventional breeding hinder its cultivation. A viable alternative for genetic improvement is the development of transgenic plants that incorporate desirable agronomic traits through the controlled insertion of genes of interest into their genome. The objective of study was to develop a protocol for obtaining transgenic plants of Agave sisalana 'RLV19,' a species widely cultivated in the semi-arid region of Bahia-BA, Brazil, using the cp4-epsps gene aimed at constitutive gene expression. Two transgenic plants of Agave sisalana 'RLV19' were regenerated via organogenesis. PCR analyses and CP4-EPSP protein expression by RT-PCR confirmed the presence and expression of the transgene in these plants. This is the first report of A. sisalana transgenic plants expressing the cp4-epsps gene, and to the best of our knowledge, there have been no prior reports on protocols for the production of transgenic plants of this species.

Indexed as

3-Phosphoshikimate 1-CarboxyvinyltransferaseAgaveBiofuelsGlycineHerbicide ResistancePlants, Genetically ModifiedGlyphosateHerbicides3-Phosphoshikimate 1-CarboxyvinyltransferaseBiofuelsGlycineGlyphosateHerbicidesAgave sisalanaBiomassHerbicide-resistantOrganogenesis

Identifiers

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.