ArticleAnalytical chemistry2025
Detection and Analysis of Reactive Oxygen Species (ROS): Buffer Components Are Not Bystanders.
Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Reactive oxygen species (ROS) research in drug discovery and drug delivery: Can the medium Be a Trojan Horse disguised as something benign?Journal of enzyme inhibition and medicinal chemistry · 2026Article
- One Stone Two Birds: Novel Fluorescent Probe Chemistry That Detects Redox Stress in Cell Culture.Analytical chemistry · 2026Article
- Conserved 4-coumarate 3-hydroxylase/ascorbate peroxidase bifunctionality coordinates lignin deposition and plant growth in Brachypodium and Populus.Plant physiology · 2026Article
- Computational redesign and directed evolution of a lanthanide-dependent photoredox enzyme for enantioselective diol cleavage.Chemical science · 2026Article
- Targeting Highly Reactive Oxygen Species (hROS) for Prodrug Activation through a Cascade Reaction with Kinetic Tunability (CReKT) to Effect Linker Cleavage.Journal of medicinal chemistry · 2026Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Reactive oxygen species (ROS) play critical roles in pathophysiological processes. Therefore, there is widespread interest in learning ROS concentrations under various conditions. However, literature numbers in ROS concentration vary significantly, and most cannot be readily compared against each other, largely because of the lack of understanding of the effects of various factors that significantly impact the experimental outcome. In this study, we examine an overlooked factor: the chemical reactivity of commonly used organic buffer molecules toward ROS and how such reactivity affects the results interpretation. Specifically, we examined HEPES, Tris, MES, citrate, ammonium acetate, and phosphate-buffered saline (PBS) and found that most organic buffer components can rapidly consume NaOCl (the second most abundant ROS) and/or directly interact with certain ROS probes such as a boronate for H
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Registered trials
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