ArticleCurrent protocols2025
Peptide-Oligonucleotide Conjugates: Catalytic Preparation in Aqueous Solution or On-Column.
Article in Current protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Peptide-Oligonucleotide Conjugates: Catalytic Preparation in Aqueous Solution or On-Column.Current protocols · 2025Article
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3 authors.
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Abstract
Peptide-oligonucleotide conjugates (POCs) are synthesized through a novel catalytic and sustainable approach. The amide coupling between peptide and oligonucleotide is facilitated by 1,4-diazabicyclo[2.2.2]octane (DABCO) as catalyst and 2-chloro-4,6-dimethoxy-1,3,5-triazine (CDMT) as a stoichiometric activating agent. Two distinct methods are described for the POC synthesis: the first involves an on-column conjugation in an anhydrous environment, while the second enables a post-synthetic conjugation in an aqueous buffer. This last approach has been successfully extended to an iterative process, offering significant potential for the development of DNA-encoded libraries.© 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: On-column conjugation of an amino acid or of a peptide to an oligonucleotide Support Protocol: Deprotection of the Boc-protected amine of the lysine Basic Protocol 2: In-solution conjugation of an amino acid or of a peptide on an oligonucleotide Basic Protocol 3: Iterative process for the preparation of a peptide-oligonucleotide conjugate.
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