Molecular identification of zoonotic taeniids metacestodes in several rodent species trapped in Central Europe.

Background: Larval stages of carnivore cestodes in rodents have been extensively studied for decades, primarily based on morphological indicators. Numerous datasets, particularly from Central Europe, exist on this topic. Traditionally, the shape, number, and size of hooks have been key distinguishing features. However, recent findings suggest that morphology alone may not provide accurate identification. In this study, rodent livers from various habitats across five regions of the Czech Republic were examined for the presence of taeniid larval cestodes. Methods: A total of 2,934 rodent specimens were collected using snap traps between 2014 and 2022. Taeniid metacestodes detected in these intermediate hosts were subsequently subjected to species determination through molecular (targeting the cytochrome oxidase subunit I gene) and morphological examinations. Results: The following cestodes were identified using molecular methods: Conclusion: This study presents the first comprehensive molecular data on the aforementioned zoonotic cestode species in the Czech Republic. The findings demonstrate the importance of using molecular techniques for Taeniidae species determination, as morphological methods may lead to erroneous classifications. Furthermore, this research highlights the importance of accurate diagnostic techniques, enabling the development of effective prevention and control strategies by verifying host-parasite relationships.