ArticleFrontiers in microbiology2025
A halophilic metalloprotease from
Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- A Study on the Effect of Copper Methionine in Alleviating Yellowing in Channel Catfish.Veterinary sciences · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purposes: This study aimed to develop a sustainable strategy for valorizing protein-rich industrial by-products into functional antioxidants using halophilic biocatalysts, addressing environmental challenges and the demand for bioactive compounds. Methods: A moderately halophilic bacterium, Results: EYHIII was a thermostable (5060°C) and alkaliphilic (pH 7.59.5) M4-family metalloprotease. The enzyme retained >80% activity under high salinity conditions (1 M NaCl) and exhibited strict substrate specificity for hydrophobic residues (Phe/Leu) at the P1' position. It efficiently hydrolyzed both soluble and insoluble collagens. Fish collagen hydrolysates generated by EYHIII demonstrated potent antioxidant activity, scavenging 33.53 ± 3.30% of DPPH radicals and 45.55 ± 3.00% of hydroxyl radicals at 3 mg/mL, with a peroxyl radical absorbance capacity of 1.69 ± 0.07 mmol TE/g. In human umbilical vein endothelial cells (HUVECs), the hydrolysate reduced high glucose-induced reactive oxygen species (ROS) to baseline levels at 200 μg/mL. It also significantly upregulated antioxidant enzymes compared to damaged controls: superoxide dismutase (SOD, 103.55%), catalase (CAT, 110.96%), and glutathione peroxidase (GSH-Px, 135.79%) (all Conclusions: This study highlighted Salinivibrio sp. YH4 and its protease EYHIII as a sustainable platform for converting collagen waste into high-value antioxidants. These findings addressed both environmental pollution and the growing demand for functional bioactive compounds. The results underscored the potential of halophilic biocatalysts in advancing circular economy strategies for protein resource utilization.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.