ArticleJDS communications2025
Effects of dietary rumen-protected choline supplementation on genome-wide DNA methylation in mammary tissue from postpartum dairy cows.
Article in JDS communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Choline is a methyl donor that may influence DNA methylation, gene expression, and cellular processes. Past studies have found an increase in milk yield when periparturient dairy cows were supplemented with dietary rumen-protected choline (RPC); however, the mechanism behind this response is unknown. Therefore, the objective of this study was to assess the effects of dietary RPC supplementation on mammary genome-wide DNA methylation. Parous Holstein cows were blocked by calving month and then randomly assigned within block to receive either 30 g/d of RPC (13.6 g/d of choline ions; CHOL30, n = 21) or no RPC (CON, n = 19) as a top-dress, starting 24 d before expected calving until 21 d postpartum. Mammary tissue was collected at d 17 postpartum and DNA was isolated. A subset of samples (n = 6 per group) was randomly selected and submitted for whole-genome bisulfite sequencing. Differentially methylated cytosines (DMC), regions, and genes (DMG) were determined using the 'genomation' R package. The cut-off values were set at false discovery rate-adjusted
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