Evidence map›Paper›PMID 40457770›Full record

ArticleInfection control and hospital epidemiology2025

Whole-genome sequencing surveillance of vancomycin-resistant

Sarah M Schrader, Meghan A Baker, Chanu Rhee, Michael Klompas, Samantha Taffner, Zachary Pearson, Jay Worley, Lynn Bry, Sanjat Kanjilal, Manfred Brigl and 1 more

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Article in Infection control and hospital epidemiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Sarah M SchraderDepartment of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
Meghan A BakerDepartment of Population Medicine, Harvard Medical School and Harvard Pilgrim Health Care Institute, Boston, MA, USA.
Chanu RheeDepartment of Population Medicine, Harvard Medical School and Harvard Pilgrim Health Care Institute, Boston, MA, USA.
Michael KlompasDepartment of Population Medicine, Harvard Medical School and Harvard Pilgrim Health Care Institute, Boston, MA, USA.
Samantha TaffnerMass General Brigham, Boston, MA, USA.
Zachary PearsonDepartment of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
Jay WorleyDepartment of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
Lynn BryDepartment of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
Sanjat KanjilalDepartment of Population Medicine, Harvard Medical School and Harvard Pilgrim Health Care Institute, Boston, MA, USA.
Manfred BriglDepartment of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
Nicole D PecoraDepartment of Pathology, Brigham and Women's Hospital, Boston, MA, USA.ORCID 0000-0001-5633-8746

Funding

STRUCTURE-FUNCTION RELATIONSHIPS IN THE ALIMENTARY TRACTP30DK034854 · NIDDK · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI JONATHAN C KAGAN · 1986 to 2026
$32.4M
NIDDK NIH HHS P30 DK034854
6 · The paper itself

Abstract

objectiveVancomycin-resistant enterococci (VRE) can cause serious healthcare-associated infections. Patients can become colonized and infected through contact with healthcare workers, hospital surfaces, equipment, and other patients. We evaluated the utility of broadly applied whole-genome sequencing (WGS) surveillance of vancomycin-resistant

designRetrospective genomic and epidemiologic analysis of clinical VREfm isolates.

settingBrigham and Women's Hospital, an 800-bed tertiary care center in Boston, MA, USA.

methodsVREfm was isolated from patient screening and diagnostic specimens. We sequenced the genomes of 156 VREfm isolates, 12 at the request of infection control and 144 as a convenience sample, and used single nucleotide polymorphism (SNP) differences to assess relatedness. For isolate pairs separated by 15 or fewer SNPs by two orthogonal comparison methods, we mapped epidemiologic connections to identify putative transmission clusters.

resultsWe found evidence for 16 putative transmission clusters comprising between two and four isolates each and involving 41/156 isolates (26.3%). Our analysis discovered 14 clusters that were missed by traditional surveillance methods and additional members of two clusters that were detected by traditional methods. Patients in four transmission clusters were linked only by exposure to the postanesthesia care unit.

conclusionsWe show that WGS surveillance for VREfm can support infection control investigations and detect transmission events missed by routine surveillance methods. We identify the postanesthesia care unit as a locus for VREfm transmission, which demonstrates how WGS surveillance could inform targeted interventions to prevent the spread of VREfm.

Indexed as

Cross InfectionDisease OutbreaksEnterococcus faeciumGram-Positive Bacterial InfectionsVancomycin-Resistant EnterococciWhole Genome SequencingBostonFemaleGenome, BacterialHumansPolymorphism, Single NucleotideRetrospective StudiesTertiary Care Centers

Identifiers

PMID40457770
PMCPMC13019461

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