ArticleRapid communications in mass spectrometry : RCM2025
Advantages of Incomplete Digestion in Human Hair Shaft Proteomics, a Focus on Cuticular Keratins.
Article in Rapid communications in mass spectrometry : RCM, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Pepsin Digestion for Proteomic Studies of the Human Hair Shaft.Rapid communications in mass spectrometry : RCM · 2026Article
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Authors and funding
4 authors.
Funding
Abstract
rationaleThe proteome of the hair shaft has been increasingly studied by mass spectrometry for sensitive, accurate, and comprehensive characterization of major hair proteins such as cuticular keratins for biomedical and forensic applications. As an external appendage of human skin, the shaft of scalp hair is formed by dead keratinized cells that are biologically and chemically stable. The extraction and digestion of hair shaft proteins have been bottlenecks in hair proteomics.
methodsWe present a straightforward and reliable sample preparation procedure using a commercial Precellys homogenizer in mild basic conditions. We further shortened the sample preparation procedure by implementing an overnight tryptic digestion for partial proteolysis instead of a 3-day complete digestion.
resultsUsing this method, we achieved over 75% protein extraction efficiency from the shaft of human scalp hair, and the limited proteolysis improved keratin sequence coverage. The robustness of our method was confirmed by high reproducibility, with R
conclusionsWe developed a facile and robust sample preparation strategy for human hair shafts. The improved sequence coverage in cuticular keratins by shortened and incomplete proteolysis is critical for the identification of genetically variant peptides in keratins.
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