Evidence map›Paper›PMID 40457455›Full record

ArticleJournal of nanobiotechnology2025

An integrated multi-mode detection platform based on CRISPR/Cas 12a and aptamers for ultra-sensitive identification of sulfamethazine and genes associated with sulfonamide resistance.

Tao Zhang, Zongwu Meng, Haoyu Yu, Zerun Zhang, Guiling Liu, Anqi Qiu, Wanshu Zheng, Ping Ding, Tianhan Kai

Abstract read
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In one paragraph

Article in Journal of nanobiotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Tao ZhangXiangya School of Public Health, Central South University, Changsha, Hunan, 410013, China.
Zongwu MengXiangya School of Public Health, Central South University, Changsha, Hunan, 410013, China.
Haoyu YuXiangya School of Public Health, Central South University, Changsha, Hunan, 410013, China.
Zerun ZhangXiangya School of Public Health, Central South University, Changsha, Hunan, 410013, China.
Guiling LiuXiangya School of Public Health, Central South University, Changsha, Hunan, 410013, China.
Anqi QiuXiangya School of Public Health, Central South University, Changsha, Hunan, 410013, China.
Wanshu ZhengXiangya School of Public Health, Central South University, Changsha, Hunan, 410013, China.
Ping DingXiangya School of Public Health, Central South University, Changsha, Hunan, 410013, China. Pingshui@csu.edu.cn.
Tianhan KaiXiangya School of Public Health, Central South University, Changsha, Hunan, 410013, China. th_kai@csu.edu.cn.

Funding

Graduate Research Innovation Program of Central South University 2025ZZTS0167the Central South University Research Program of Advanced Interdisciplinary Studies 2023QYJC013the National Natural Science Foundation of China 82373635the Science and Technology Innovation Program of Hunan Province 2022RC1206
6 · The paper itself

Abstract

The production and buildup of sulfamethazine (SMZ) and resistance genes for sulfonamide antibiotics (sul1) pose a serious risk to environmental and public health safety. Creating advanced sensing systems that are both highly sensitive and selective for the prolonged observation of SMZ concentrations in the environment, along with the quantification of sul1 gene prevalence, aims to identify trends in resistance, posing a considerable challenge. Here, we devised a platform (SMZ-sul1 multi-mode detection platform) that allows for the fluorescence detection of SMZ in environmental samples. This is achieved through the competition for the aptamer between the complementary base and SMZ, along with the colorimetric, photothermal, and electrochemical tracking of sul1, using a magnetic separation unit (FP@cDNA). MOF-818@PtPd (MPP) nanozymes with high peroxide mimetic enzyme activity were linked to FP@cDNA through Zr-O-P bond and employed as a catalyst for the 3,3',5,5'-tetramethylbenzidine (TMB) oxidation, as well as for electrocatalytic hydrogen peroxide (H

Indexed as

Anti-Bacterial AgentsAptamers, NucleotideCRISPR-Cas SystemsDrug Resistance, BacterialSulfamethazineSulfonamidesBenzidinesBiosensing TechniquesElectrochemical TechniquesLimit of DetectionMetal-Organic Frameworks3,3',5,5'-tetramethylbenzidineAnti-Bacterial AgentsAptamers, NucleotideBenzidinesMetal-Organic FrameworksSulfamethazineSulfonamidesAptamerCRISPR/Cas12aMagnetic separationMulti-mode detectionNanozymeSMZSul1

Identifiers

PMID40457455
PMCPMC12131764

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.