Evidence map›Paper›PMID 40456842›Full record

ArticleNature communications2025

Wastewater surveillance of SARS-CoV-2 from aircraft to citywide monitoring.

Mariel Perez-Zabaleta, Carlo Berg, Neus Latorre-Margalef, Isaac Owusu-Agyeman, Ayda Kiyar, Helene Botnen, Caroline Schönning, Luisa W Hugerth, Zeynep Cetecioglu

Abstract read
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Mariel Perez-ZabaletaDepartment of Industrial Biotechnology, School of Engineering Sciences in Chemistry Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden.
Carlo BergDepartment of Microbiology, Public Health Agency of Sweden, Solna, Sweden.ORCID http://orcid.org/0000-0001-7815-1077
Neus Latorre-MargalefDepartment of Communicable Disease Control and Health Protection, Public Health Agency of Sweden, Solna, Sweden.
Isaac Owusu-AgyemanDepartment of Industrial Biotechnology, School of Engineering Sciences in Chemistry Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden.
Ayda KiyarDepartment of Industrial Biotechnology, School of Engineering Sciences in Chemistry Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden.
Helene BotnenDepartment of Communicable Disease Control and Health Protection, Public Health Agency of Sweden, Solna, Sweden.ORCID http://orcid.org/0009-0008-1027-0676
Caroline SchönningDepartment of Communicable Disease Control and Health Protection, Public Health Agency of Sweden, Solna, Sweden.
Luisa W HugerthDepartment of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden. luisa.hugerth@scilifelab.se.ORCID http://orcid.org/0000-0001-5432-1764
Zeynep CeteciogluDepartment of Industrial Biotechnology, School of Engineering Sciences in Chemistry Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden. zeynepcg@kth.se.ORCID http://orcid.org/0000-0002-8170-379X

Funding

Folkhälsomyndigheten (Public Health Agency of Sweden) 05204-2022Folkhälsomyndigheten (Public Health Agency of Sweden) S2022/04841
6 · The paper itself

Abstract

Wastewater monitoring is highly efficient in SARS-CoV-2 surveillance for tracking virus spread through travel, surpassing traditional airport passenger testing. This study explored the links between SARS-CoV-2 contents and variants from aircraft to city, assessing the impact of detected variants from international travellers versus the local population. A total of 969 variants using next-generation sequencing (NGS) were examined to understand the links between-aircraft, Arlanda airport, wastewater treatment plants (WWTPs), and Stockholm city-and compared these to variants detected in Stockholm hospitals from January to May 2023. SARS-CoV-2 contents in WWTPs reflected local infection rates, requiring analysis from multiple plants for an accurate city-wide infection assessment. Variants initially detected in aircraft arriving from China did not spread widely during the study period. RT-qPCR is adequate for the detection of specific variants in wastewater, including Variants Under Monitoring. However, NGS remains a powerful method for identifying novel variants. Wastewater monitoring was more effective than clinical testing in the early detection of specific variants, with notable delays observed in clinical surveillance. Furthermore, a broad range of variants are detected in wastewater that surpasses clinical tests. This underscores the vital role of wastewater-based epidemiology in managing future outbreaks and enhancing global health security.

Indexed as

AircraftCOVID-19SARS-CoV-2WastewaterWastewater-Based Epidemiological MonitoringAirportsChinaCitiesHigh-Throughput Nucleotide SequencingHumansSwedenTravelWastewater

Identifiers

PMID40456842
PMCPMC12130461

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.