Evidence map›Paper›PMID 40455335›Full record

ArticlePlant cell reports2025

Enhanced thermotolerance via overexpression of a stromal ascorbate peroxidase in Nannochloropsis oceanica.

Shan Wu, Yiting She, Xitao Chen, Xingwei Huang, Tao Xu, Congcong Miao, Qianyi Wu, Zheng Li, Chengcheng Li, Yuhui Cheng and 1 more

Abstract read
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Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Shan Wu *School of Marine Biology and Fisheries, Hainan University, Haikou, 570228, China.
Yiting She *Faculty of Biology and Biotechnology, Photobiotechnology, Ruhr-University Bochum, 44801, Bochum, Germany.
Xitao ChenSchool of Marine Biology and Fisheries, Hainan University, Haikou, 570228, China.
Xingwei HuangSchool of Marine Biology and Fisheries, Hainan University, Haikou, 570228, China.
Tao XuSchool of Marine Biology and Fisheries, Hainan University, Haikou, 570228, China.
Congcong MiaoSchool of Marine Biology and Fisheries, Hainan University, Haikou, 570228, China.
Qianyi WuSchool of Marine Biology and Fisheries, Hainan University, Haikou, 570228, China.
Zheng LiSchool of Marine Biology and Fisheries, Hainan University, Haikou, 570228, China.
Chengcheng LiSchool of Marine Biology and Fisheries, Hainan University, Haikou, 570228, China.
Yuhui ChengSchool of Marine Biology and Fisheries, Hainan University, Haikou, 570228, China.
Yi XinSchool of Marine Biology and Fisheries, Hainan University, Haikou, 570228, China. xinyi@hainanu.edu.cn.ORCID http://orcid.org/0000-0002-5686-2967

Funding

Open Project of State Key Laboratory of Marine Resource Utilization in South China Sea DC2300001799Start-Up Funds of Hainan University RZ2300002678the National Key R&D Program of China 2018YFA0902500
6 · The paper itself

Abstract

key messageOverexpression of the heat-responsive ascorbate peroxidase gene NoAPX2430 improves thermotolerance and growth of Nannochloropsis oceanica, offering potential strategies for enhanced resilience in plant cells. Microalgae are promising for industrial lipid production but face challenges from high-temperature (HT) stress. This study focused on improving thermotolerance in Nannochloropsis oceanica by studying and modifying the heat-responsive ascorbate peroxidase gene, NoAPX2430. Functional and localization analyses confirmed NoAPX2430 as an active enzyme in the chloroplast stroma. Knockdown of NoAPX2430 resulted in reduced growth, photosynthetic efficiency, and reactive oxygen species (ROS) scavenging under HT (35 °C). In contrast, overexpression of NoAPX2430 significantly improved photosynthetic performance, lowered ROS levels, and boosted growth rates. Additionally, lipid and triacylglycerol contents increased significantly under combined nitrogen depletion and HT, with polyunsaturated fatty acids rising by up to 194.4%. These results highlight NoAPX2430's critical role in enhancing thermotolerance and lipid biosynthesis. This provides a foundation for genetic and environmental strategies to boost microalgal resilience and productivity for sustainable biofuel and nutraceutical development.

Indexed as

Ascorbate PeroxidasesMicroalgaeStramenopilesThermotoleranceChloroplastsPhotosynthesisReactive Oxygen SpeciesAscorbate PeroxidasesReactive Oxygen SpeciesAscorbate peroxidaseLipidMicroalgaeNannochloropsisThermotolerance

Identifiers

PMID40455335

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.